BT 207 |
Genetic Engineering |
3-0-0-6 |
Pre-requisites
: Nil Syllabus The basis of genetic engineering: Genome
organization; the flow of genetic information; Genes and genomes; Genome size
and complexity; The transcriptome and proteome. Labelling
nucleic acids; End labelling; Nick translation; Labelling by primer
extension; Nucleic acid hybridization. DNA sequencing; Principles of DNA
sequencing; Next-Gen sequencing. Human Genome
Project. Techniques in Genetic Engineering: DNA/ RNA
extraction, Polymerase Chain reaction: primer design, Nested PCR, inverse
PCR, RAPD, Real-time PCR. Gel Electrophoresis-DNA, RNA and Protein. Blotting:
DNA, RNA and Protein. Site directed mutagenesis, DNA/ Protein interactions:
CHIP, EMSA, Reporter assays, pull down assays. DNA modifying enzymes and vectors: Nucleases;
Polymerases; Enzymes that modify the ends of DNA molecules: Terminal Transferase, T4 Polynucleotide Kinase, Alkaline
Phosphatases; DNA ligase, Restriction enzymes. Vectors for cloning and
expression in prokaryotes and Eukaryotes: Plasmid Vectors, Phage Vectors, Cosmids, Phagemids, BACs, Yeast Vectors, YACs, Lentiviral
Vectors, Adenoviral Vectors, Plant Vectors, Insect Vectors. In-frame protein fusion and use of GFP for subcellular localization of protein/DNA. cDNA and genomic DNA library
construction. Introduction of foreign DNA: Transfection, transformation,
particle gun and Ti plasmid mediated. Recent advances and applications: ZFNs,
TALENs, CRISPR/Cas systems. Transgenic plants and
animals, gene therapy, forensic applications. Bioinformatics: DNA and protein databases.
Promoter identification, Gene expression analysis, Big data analysis. Text
Books 1. Desmond S. T. Nicholl,
An Introduction to Genetic Engineering (3rd Ed),
Cambridge University Press, 2010. 2. T. A. Brown, Gene Cloning and DNA Analysis: An
Introduction, (6th Ed), Wiley-Blackwell, 2012. 3. Sandy B. Primrose, Richard Twyman,
Principles of Gene Manipulation and Genomics, (8th Ed), Blackwell Publishing,
2016 4. Michael R. Green and Joseph Sambrook, Molecular Cloning: A Laboratory Manual, (4th
Ed), Cold Spring Harbor Laboratory Press, 2012. References 1. Bernard R. Glick, Jack J. Pasternak, Cheryl L.
Patten, Molecular Biotechnology: Principles and Applications of Recombinant
DNA (4th Ed), ASM Press, 2010. 2. T.A. Brown, Introduction to Genetics: A
Molecular Approach, (1st Ed), Garland Science, 2011. 3. Jordanka Zlatanova, Kensal van Holde, Molecular
Biology, (1st Ed), 201 |