BTech Course Structure and Syllabus for
Biotechnology (To
be applicable from 2010 batch onwards) |
||||||||||||
Course
No. |
Course Name |
L |
T |
P |
C |
|
Course
No. |
Course Name |
L |
T |
P |
C |
Semester - 1 |
|
Semester -2 |
||||||||||
CH101 |
Chemistry |
3 |
1 |
0 |
8 |
|
BT101 |
Modern
Biology |
3 |
0 |
0 |
6 |
EE101 |
Electrical
Sciences |
3 |
1 |
0 |
8 |
|
CS
101 |
Introduction
to Computing |
3 |
0 |
0 |
6 |
MA101 |
Mathematics
- I |
3 |
1 |
0 |
8 |
|
MA102 |
Mathematics
- II |
3 |
1 |
0 |
8 |
PH101 |
Physics
- I |
2 |
1 |
0 |
6 |
|
ME101 |
Engineering
Mechanics |
3 |
1 |
0 |
8 |
CH110 |
Chemistry
Laboratory |
0 |
0 |
3 |
3 |
|
PH102 |
Physics
- II |
2 |
1 |
0 |
6 |
ME110/ PH 110 |
Workshop
/Physics Laboratory |
0 |
0 |
3 |
3 |
|
CS110 |
Computing
Laboratory |
0 |
0 |
3 |
3 |
ME
111 ** |
Engineering
Drawing |
0 |
0 |
3 |
3 |
|
EE102 |
Basic
Electronics Laboratory |
0 |
0 |
3 |
3 |
SA
101 |
Physical
Training -I |
0 |
0 |
2 |
0 |
|
PH110/
ME 110 |
Physics
Laboratory/Workshop |
0 |
0 |
3 |
3 |
NCC/NSO/NSS |
0 |
0 |
2 |
0 |
|
SA
102 |
Physical
Training -II |
0 |
0 |
2 |
0 |
|
12 |
4 |
9 |
41 |
|
|
NCC/NSO/NSS |
0 |
0 |
2 |
0 |
||
** For 2010 batch the credit structure is 0-0-3-3 |
|
|
|
14 |
3 |
9 |
43 |
|||||
Semester 3 |
|
Semester 4 |
||||||||||
MA201 |
Mathematics-
III |
3 |
1 |
0 |
8 |
|
BT202 |
Microbiology |
3 |
0 |
0 |
6 |
BT201 |
Biochemistry |
3 |
0 |
0 |
6 |
|
BT205 |
Biophysics |
2 |
1 |
0 |
6 |
CL201
|
Chemical
Process Calculations |
2 |
1 |
0 |
6 |
|
BT208 |
Molecular
Biology & Genetic Engineering |
3 |
1 |
0 |
8 |
CL202
|
Fluid
Mechanics |
3 |
1 |
0 |
8 |
|
CL205 |
Mass
Transfer Operation - I |
2 |
1 |
0 |
6 |
CL203
|
Chemical
Engineering Thermodynamics -I |
2 |
1 |
0 |
6 |
|
HS2xx |
HSS
Elective - II |
3 |
0 |
0 |
6 |
HS2xx |
HSS
Elective - I |
3 |
0 |
0 |
6 |
|
BT290 |
Biomolecular
Analysis Laboratory |
0 |
0 |
6 |
6 |
SA201 |
Physical
Training - III |
0 |
0 |
2 |
0 |
|
SA202 |
Physical
Training - IV |
0 |
0 |
2 |
0 |
NCC/NSO/NSS |
0 |
0 |
2 |
0 |
|
|
NCC/NSO/NSS |
0 |
0 |
2 |
0 |
|
16 |
4 |
0 |
40 |
|
|
|
13 |
3 |
6 |
38 |
||
Semester 5 |
|
Semester 6 |
||||||||||
BT301 |
Bioinformatics
and Computational Biology |
2 |
0 |
0 |
4 |
|
BT302 |
Biochemical
Engineering |
3 |
1 |
0 |
8 |
BT303 |
Immunology |
3 |
0 |
0 |
6 |
|
BT305 |
Industrial
Microbiology |
3 |
0 |
0 |
6 |
BT309 |
Plant
Biotechnology |
3 |
0 |
0 |
6 |
|
BT306 |
IPR,
Ethics and Bio-safety |
3 |
0 |
0 |
6 |
CL303
|
Chemical
Reaction Engineering - I |
2 |
1 |
0 |
6 |
|
BT308 |
Animal
Cell Biotechnology |
3 |
0 |
0 |
6 |
HS3xx |
HSS
Elective -III |
3 |
0 |
0 |
6 |
|
BTxxx |
Departmental
Elective - I |
3 |
0 |
0 |
6 |
BT380 |
Molecular
Biotechnology Laboratory |
0 |
0 |
6 |
6 |
|
BT330 |
Biochemical
Engineering Laboratory |
0 |
0 |
6 |
6 |
BT310 |
Bioinformatics
and Computational Biology Lab |
0 |
0 |
6 |
6 |
|
|
|
15 |
1 |
6 |
38 |
13 |
1 |
12 |
40 |
|
|
|
|
|
|
|
||
Semester 7 |
|
Semester 8 |
||||||||||
BT404 |
Bioseparation
Engineering |
3 |
0 |
0 |
6 |
|
BT401 |
Frontiers
in Biotechnology |
3 |
0 |
0 |
6 |
BT405 |
Environmental
Biotechnology |
3 |
0 |
0 |
6 |
|
BTxxx |
Departmental
Elective - IV |
3 |
0 |
0 |
6 |
BTxxx |
Departmental
Elective - II |
3 |
0 |
0 |
6 |
|
BTxxx |
Departmental
Elective - V |
3 |
0 |
0 |
6 |
BTxxx |
Departmental
Elective - III |
3 |
0 |
0 |
6 |
|
HS4xx |
HSS
Elective - IV |
3 |
0 |
0 |
6 |
XXxxx |
Open
Elective - I |
3 |
0 |
0 |
6 |
|
XXxxx |
Open
Elective - II |
3 |
0 |
0 |
6 |
BT
498 |
Project
-I |
0 |
0 |
6 |
6 |
|
BT
499 |
Project
-II |
0 |
0 |
6 |
6 |
15 |
0 |
6 |
36 |
|
|
|
15 |
0 |
6 |
36 |
CH 101 Chemistry (3-1-0-8) Structure
and Bonding; Origin of quantum theory, postulates of quantum mechanics; Schrodinger
wave equation: operators and observables, superposition theorem and
expectation values, solutions for particle in a box, harmonic oscillator,
rigid rotator, hydrogen atom; Selection rules of microwave and vibrational
spectroscopy; Spectroscopic term symbol; Molecular orbitals: LCAO-MO; Huckel
theory of conjugated systems; Rotational, vibrational and electronic
spectroscopy; Chemical Thermodynamics: The zeroth and first law, Work, heat,
energy and enthalpies; The relation between Cv and Cp;
Second law: entropy, free energy (the Helmholtz and Gibbs) and chemical
potential; Third law; Chemical equilibrium; Chemical kinetics: The rate of
reaction, elementary reaction and chain reaction; Surface: The properties of
liquid surface, surfactants, colloidal systems, solid surfaces, physisorption
and chemisorption; The periodic table of elements; Shapes of inorganic
compounds; Chemistry of materials; Coordination compounds: ligand,
nomenclature, isomerism, stereochemistry, valence bond, crystal field and molecular
orbital theories; Bioinorganic chemistry and organometallic chemistry; Stereo
and regio-chemistry of organic compounds, conformers; Pericyclic reactions;
Organic photochemistry; Bioorganic chemistry: Amino acids, peptides,
proteins, enzymes, carbohydrates, nucleic acids and lipids; Macromolecules
(polymers); Modern techniques in structural elucidation of compounds (UV-vis,
IR, NMR); Solid phase synthesis and combinatorial chemistry; Green chemical
processes. Texts:
1. P. W. Atkins, Physical Chemistry, 5th Ed., ELBS, 1994. 2. C.
N. Banwell, and E. M. McCash, Fundamentals
of Molecular Spectroscopy, 4th Ed., Tata McGraw-Hill, 1962. 3. F.
A. Cotton, and G. Wilkinson, Advanced
Inorganic Chemistry, 3rd Ed., Wiley Eastern Ltd., New Delhi,
1972, reprint in 1988. 4. D. J. Shriver, P. W. Atkins, and C. H.
Langford, Inorganic Chemistry, 2nd
Ed., ELBS ,1994. 5. S. H. Pine, Organic Chemistry, McGraw-Hill, 5th Ed., 1987 References: 1. I. A. Levine, Physical Chemistry, 4th Ed., McGraw-Hill, 1995. 2. I. A. Levine, Quantum Chemistry, EE Ed., prentice Hall, 1994. 3. G. M. Barrow, Introduction to Molecular Spectroscopy, International Edition,
McGraw-Hill, 1962 4. J.
E. Huheey, E. A. Keiter and R. L. Keiter, Inorganic
Chemistry: Principle, structure and reactivity, 4th Ed.,
Harper Collins, 1993 5. L. G. Wade (Jr.), Organic Chemistry, Prentice Hall, 1987. |
CS 101
Introduction to Computing (3-0-0-6)
Introduction:
The von Neumann architecture, machine language, assembly language, high level
programming languages, compiler, interpreter, loader, linker, text editors,
operating systems, flowchart; Basic features of programming (Using C): data
types, variables, operators,
expressions, statements, control structures, functions; Advanced
programming features: arrays and pointers, recursion, records (structures),
memory management, files, input/output, standard library functions,
programming tools, testing and debugging; Fundamental operations on data:
insert, delete, search, traverse and modify; Fundamental data structures:
arrays, stacks, queues, linked lists; Searching and sorting: linear search,
binary search, insertion-sort, bubble-sort, selection-sort, radix-sort,
counting-sort; Introduction to object-oriented programming Texts:
1. A Kelly and I Pohl, A Book on C, 4th Ed.,
Pearson Education, 1999. 2. A M Tenenbaum, Y Langsam and M J
Augenstein, Data Structures Using C,
Prentice Hall India, 1996. References: 1.
H Schildt, C: The Complete Reference,
4th Ed., Tata Mcgraw Hill, 2000 2. B Kernighan and
D Ritchie, The C Programming Language,
4th Ed., Prentice Hall of India, 1988. |
CS 110 Computing
Laboratory (0-0-3-3)
Programming
Laboratory will be set in consonance with the material covered in CS101. This
will include assignments in a programming language like C. References: 1.
B. Gottfried and J. Chhabra,
Programming With C, Tata Mcgraw Hill, 2005 MA 102 Mathematics
- II
(3-1-0-8) Vector functions of one variable –
continuity and differentiability; functions of several variables –
continuity, partial derivatives, directional derivatives, gradient,
differentiability, chain rule; tangent planes and normals, maxima and minima,
Lagrange multiplier method; repeated and multiple integrals with applications
to volume, surface area, moments of inertia, change of variables; vector
fields, line and surface integrals; Green’s, Gauss’ and
Stokes’ theorems and their applications. First order differential equations – exact
differential equations, integrating factors, Bernoulli equations, existence
and uniqueness theorem, applications; higher-order linear differential
equations – solutions of homogeneous and nonhomogeneous equations,
method of variation of parameters, operator method; series solutions of
linear differential equations, Legendre equation and Legendre polynomials,
Bessel equation and Bessel functions of first and second kinds; systems of
first-order equations, phase plane, critical points, stability. Texts: 1.
G. B. Thomas (Jr.) and R. L. Finney, Calculus and Analytic Geometry, 9th
Ed., Pearson Education India, 1996. 2.
S. L. Ross, Differential Equations, 3rd Ed., Wiley India,
1984. References: 1. T.
M. Apostol, Calculus - Vol.2, 2nd
Ed., Wiley India, 2003. 2. W.
E. Boyce and R. C. DiPrima, Elementary
Differential Equations and Boundary Value Problems, 9th Ed.,
Wiley India, 2009. 3. E.
A. Coddington, An Introduction to
Ordinary Differential Equations, Prentice Hall India, 1995. 4. E.
L. Ince, Ordinary Differential
Equations, Dover Publications, 1958. ME
101 Engineering
Mechanics (3-1-0-8) Basic principles:
Equivalent force system; Equations of equilibrium; Free body diagram;
Reaction; Static indeterminacy. Structures: Difference between trusses, frames
and beams, Assumptions followed in the analysis of structures; 2D truss;
Method of joints; Method of section;
Frame; Simple beam; types
of loading and supports; Shear
Force and bending Moment diagram in beams; Relation among load, shear force
and bending moment. Friction: Dry friction; Description and applications of
friction in wedges, thrust bearing (disk friction), belt, screw, journal
bearing (Axle friction); Rolling resistance. Virtual work and Energy method:
Virtual Displacement; Principle of virtual work; Applications of virtual work
principle to machines; Mechanical efficiency; Work of a force/couple (springs
etc.); Potential energy and equilibrium; stability. Center of Gravity and
Moment of Inertia: First and second moment of area; Radius of gyration; Parallel axis theorem; Product of inertia, Rotation of axes
and principal moment of inertia;
Moment of inertia of simple and composite bodies. Mass moment of
inertia. Kinematics of Particles: Rectilinear motion; Curvilinear motion; Use
of Cartesian, polar and spherical coordinate system; Relative and constrained
motion; Space curvilinear motion. Kinetics of Particles: Force, mass and
acceleration; Work and energy; Impulse and momentum; Impact problems; System
of particles. Kinematics and Kinetics of Rigid Bodies: Translation; Fixed
axis rotational; General plane
motion; Coriolis acceleration;
Work-energy; Power; Potential energy; Impulse-momentum and associated
conservation principles; Euler
equations of motion and its application. Texts 1. I. H. Shames, Engineering Mechanics:
Statics and Dynamics, 4th Ed., PHI, 2002. 2.
F. P. Beer and E. R. Johnston, Vector Mechanics for Engineers, Vol I - Statics, Vol II – Dynamics,
3rd Ed., Tata McGraw Hill, 2000. References 1. J.
L. Meriam and L. G. Kraige, Engineering Mechanics, Vol I – Statics, Vol II –
Dynamics, 5th Ed., John
Wiley, 2002. 2. R. C. Hibbler, Engineering Mechanics,
Vols. I and II, Pearson Press,
2002. PH 102 Physics
- II
(2-1-0-6) Vector Calculus: Gradient, Divergence and Curl,
Line, Surface, and Volume integrals, Gauss's divergence theorem and Stokes'
theorem in Cartesian, Spherical polar, and Cylindrical polar coordinates,
Dirac Delta function. Electrostatics: Gauss's law and its
applications, Divergence and Curl of Electrostatic fields, Electrostatic
Potential, Boundary conditions, Work and Energy, Conductors, Capacitors,
Laplace's equation, Method of images, Boundary value problems in Cartesian
Coordinate Systems, Dielectrics, Polarization, Bound Charges, Electric displacement,
Boundary conditions in dielectrics, Energy in dielectrics, Forces on
dielectrics. Magnetostatics: Lorentz force, Biot-Savart and
Ampere's laws and their applications, Divergence and Curl of Magnetostatic
fields, Magnetic vector Potential, Force and torque on a magnetic dipole,
Magnetic materials, Magnetization, Bound currents, Boundary conditions. Electrodynamics: Ohm's law, Motional EMF,
Faraday's law, Lenz's law, Self and Mutual inductance, Energy stored in
magnetic field, Maxwell's equations, Continuity Equation, Poynting Theorem,
Wave solution of Maxwell Equations. Electromagnetic waves: Polarization, reflection
& transmission at oblique incidences. Texts:
References:
EE 102 Basic Electronics Laboratory (0-0-3-3) Experiments using diodes and
bipolar junction transistor (BJT): design and analysis of half -wave and
full-wave rectifiers, clipping circuits and Zener regulators, BJT
characteristics and BJT amplifiers; experiments using operational amplifiers
(op-amps): summing amplifier, comparator, precision rectifier, astable and
monostable multivibrators and oscillators; experiments using logic gates:
combinational circuits such as staircase switch, majority detector, equality
detector, multiplexer and demultiplexer; experiments using flip-flops:
sequential circuits such as non-overlapping pulse generator, ripple counter,
synchronous counter, pulse counter and numerical display.
3.
R.J. Tocci, Digital Systems, 6th Ed., 2001. |
BT 201 Biochemistry (3-0-0-6) Basic
concept and design of metabolism; carbohydrate metabolism: glycolysis,
gluconeogenesis, citric acid cycle, pentose phosphate pathway, glycogen
metabolism, oxidative phosphorylation; photosynthesis; fatty acid metabolism;
protein: synthesis, targeting and turnover; biosynthesis of amino acids and
nucleotides; Integration of metabolisms; hormones; enzymes: structure, mechanism
and reaction kinetics; introduction to information metabolism. Texts:
1.
D. L. Nelson and M. M. Cox, Lehninger Principles of Biochemistry,
5th Ed., Macmillan Worth, 2007. 2.
J. L. Tymoczko, J. M. Berg and L.
Stryer, Biochemistry, 5th
Ed., W. H. Freeman, 2002. References:
1.
W. W. Parson, D. E. Vance and G. L.
Zubay, Principles of Biochemistry,
Wm. C. Brown Publishers, 1995. 2.
K. E. Van Holde, C. K. Mathews and
K. G. Ahern, Biochemistry, Pearson
Education, 2000. 3.
R. K. Murray, D. K. Granner, P. A.
Mayes and V. W. Rodwell, Harper’s
Biochemistry, McGraw Hill, 2002. |
BT 202 Microbiology
(3-0-0-6) Microbial
cell structure and function; fundamentals of microbial taxonomy and
diversity; molecular tools in microbial taxonomy; microscopic techniques;
microbial nutrition; growth and control; microbial metabolism; mutations and
DNA repair; plasmids; transformation; conjugation; transduction; transposons;
fundamentals of gene regulation; fundamentals of microbial genomics;
microbial pathogenicity and diseases. Texts:
1. G.
Tortora, B. Funke and C. Case, Microbiology,
An Introduction (International Edition), 8th Ed., Pearson
Education, 2003. 2. M.
Madigan, J. Martinko and J. Parker, Brock’s
Biology of Microorganisms, 10th Ed., Prentice Hall,
2002. References:
1. R.
Y. Stanier, J. L. Ingraham, M.L. Wheelis and P. R. Painter, General Microbiology, 5th
Ed., Macmillan Press, 1987. 2. L.
M. Prescott, J. P. Harley and D. A. Klein, Microbiology, 6th Ed., McGraw Hill, 2005. 3. J.
G. Black, Microbiology: Principles
& Explorations, 5th Ed., John Wiley & Sons Inc., 2002. 4. Benjamin
Lewin, Genes VIII, Pearson
Education, International Edition, 2004. |
BT 205 Biophysics
(2-1-0-6) Structure
and structural dynamics of DNA, RNA and proteins; techniques for monitoring
structure and dynamics: absorption, fluorescence, circular dichroism, light
scattering; methods for separation and characterization of molecules;
size-exclusion chromatography; electrophoresis; MALDI–TOF; ESI–quadrupole
mass spectrometry; thermodynamics and equilibria of macromolecules in
solution; biophysics of membranes; photobiological processes; nerve impulse;
muscle contraction; modelling biological processes; protein folding and
aggregation; protein function (Myosin and Kinesin). Texts: 1. K.
E. van Holde, W. C. Johnson and P. S. Ho, Principles
of Physical Biochemistry, Prentice Hall, 1998. 2. C.
R. Cantor and P. R. Schimmel, Biophysical
Chemistry (Parts I, II and III),
W. H. Freeman and Co., 1980. Reference:
1.
K. A. Dill and S. Bromberg, Molecular
Driving Forces. Statistical thermodynamics in Chemistry and Biology,
Garland Science, 2003. |
BT 208
Molecular Biology & Genetic
Engineering (3-1-0-8) Cell
organization and subcellular structure; structure and properties of nucleic
acids; organization of prokaryotic and eukaryotic genomes; mechanisms of DNA
replication; mutagenesis and processes of DNA repair; transcription;
translation; mechanisms of DNA recombination; regulation of gene expression;
eukaryotic RNA splicing and processing; cell cycle; programmed cell death;
cell transformation; genes in differentiation and development; oncogenes.
genetic engineering: restriction modification enzymes; cloning vectors:
plasmids, phages, cosmids, phagemids, yeast and bacterial artificial
chromosomal vectors; construction cDNA and genomic libraries; screening of
libraries: by DNA hybridization, immuno and protein assays; gene cloning and
expression in prokaryotes and eukaryotes; recombinant protein expression in
E. coli, yeast and baculovirus; mammalian cell expression vectors (Selectable
markers, Two-hybrid expression system); chimeric vectors; Site-directed
mutagenesis and its applications; transposons, gene targeting; site specific
recombination; polymerase chain reaction (PCR); applications of reverse
transcription PCR (RT-PCR) and real time PCR; principles and applications of
DNA finger printing; gene mapping by restriction fragment length polymorphism
(RFLP); application of differential display and subtractive
hybridization. Texts:
1. B.
Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts and P. Walter, Molecular Biology of Cell, 4th
Ed., Garland Publishing, 2002. 2. H.
Lodish, A. Berk, S. L. Zipursky, M. P. Scott and J. Darnell, Molecular Cell Biology, 4th
Ed., W. H. Freeman & Co., 2003.
References:
1.
B. Lewin, Genes VIII, International
Edition, Pearson Education, 2004. 2.
B. R. Glick and J. J. Pasternak, Molecular
Biotechnology: Principles and Applications of Recombinant DNA, 3rd
Ed., ASM Press, 2003. 3. R.
M. Twyman, S. B. Primrose and R. W. Old, Principles
of Gene Manipulation, Blackwell Science, 2001. |
BT 290 Biomolecular
Analysis Laboratory (0-0-6-6) Theory, operation and handling of instruments to be used in
this Lab course; Estimation of DNA in solution; Estimation of protein in
solution; Estimation of carbohydrate; Enzyme Linked Immuno Assay;
Purification of protein by chromatography; Equilibrium unfolding of a
protein; Gel electrophoresis of protein; Study of Enzyme kinetics. Texts: 1.
R. Boyer, Modern Experimental
Biochemistry, 3rd
Ed., Pearson Education (Singapore) Pvt. Ltd., 2001. 2. R. L. Switzer and L. F. Garrity, Experimental Biochemistry, 3rd Ed., W. H. Freeman, 1999. Reference: 1.
K. Wilson and J. Walker (ed.), Practical
Biochemistry, Principles and Techniques, Cambridge University Press,
1995. |
BT 301 Bioinformatics and Computational Biology (2-0-0-4) Introduction
to bioinformatics; Gene bank sequence database; submitting sequences to
database; Analysis of genome content and organization; Analysis of protein content
and organization; Analysis of protein structures; Identification of signature
motifs in proteins; Secondary structure prediction; Comparative genomics and
proteomics, Basics of aligning nucleic acid and protein sequences;
Phylogenetic analysis using internet; protein structure-function
relationships; computational analysis of protein-ligand binding; enzyme
catalysis and protein folding. Texts:
1.
D. Baxevanis, and B.
F. F. Ouellette, Bioinformatics –
A Practical Guide to the Analysis of Genes and Proteins, 2nd
Ed., John Wiley and Sons Inc., 2001. 2. A. R. Leach, Molecular Modelling: Principles and
Applications, Addison-Wesley Pub. Co. 1997. References:
1.
P. E. Bourne and H. Weissig, Structural
Bioinformatics, WILEY, 2003. 2.
T. Lengauer, Bioinformatics - From
Genomes to Drugs, Vols 1 and 2, Wiley-VCH, 2002. |
BT 303 Immunology (3-0-0-
6) Introduction
to immune system; evolution of immunity; elements of Immune system; cell
migration & inflammation; immunogens & antigens; antibody structure
and function – catalytic Antibodies, antigen antibody interactions,
humoral and cellular immunity – MHC, antibody diversity, dendritic
cells (APC), control mechanisms in the immune response cytokines –
complement – autoimmunity; immunity to different types of pathogens,
vaccination; tumor immunology; immune diseases and disorders; transplantation
immunology; co-stimulatory pathways; hybridoma and immunoassays. Texts: 1. I. Roitt, J. Brostoff and D. Male, Immunology, 6th Ed.,
Harcourt Publishers, 2001. 2. R. A. Goldsby, T. J. Kindt, B. A.
Osborne and J. Kuby, Immunology, W.
H. Freeman & Co, 2003. References:
1. A. K.
Abbas, A. H. Lichtman and J. S. Pober, Cellular
and Molecular Immunology, W. B. Saunders Co., 2000. 2. D. M.
Weir and J. Stewart, Immunology,
Churchill Livingstone, 1997. 3. A. Cooke, M. Owen, J. Trowsdale, B.
Champion and D. K. Male, Advanced
Immunology, Mosby Publ., 1996. 4.
R. Coico, G. Sunshine and E. Benjammini, Immunology: A short Course, Wiley-Liss Publ., 2003. |
BT 309 Plant
Biotechnology (3-0-0-6) Plant
morphogenesis; cellular totipotency;
in vitro culture; protoplast isolation
and culture; somatic hybridization;
haploid and triploid
production; somaclonal variation; embryo rescue and synthetic seeds; production of secondary metabolites;
cryopreservation and conservation of germplasm; plant gene structure,
function and regulation; plant transformation; marker genes; promoters;
molecular analysis; chloroplast transformation; genetic engineering for
resistance to insects, pests, virus, pathogens and tolerance to herbicides;
gene silencing; metabolic engineering; molecular farming; molecular markers
for plant improvement; plant
genomics. Texts: 1. A. Slater, N. Scott and M. Fowler, Plant Biotechnology: The genetic
manipulation of plants, Oxford University Press, 2003.
2. S.
S. Bhojwani and M. K. Razdan, Plant
Tissue Culture: Theory and Practice, Elsevier, 1996. . References:
1. J.
Hammond, P. McGarvey and V. Yusibov, Plant
Biotechnology: New Products and
Applications, Springer Verlag, 1999. 2. P.
Jones, P. J. Jones and J. M. Sutton, Plant
Molecular Biology: Essential Techniques, John Wiley & Sons, 1997. 3. Potrykus
and G. Spangenberg, Gene Transfer to
Plants, Springer Verlag, 1995. |
BT 380 Molecular
Biotechnology Laboratory (0-0-6-6) Theory, operation and handling of instruments to be used in
this Lab course; Aseptic Techniques for plant and microbial culture; In vitro
plant regeneration by Organogenesis, Somatic embryogenesis, Meristem and
Nodal segment Culture; Meiotic and Mitotic Chromosome preparation; Isolation
of pure microbial culture and quantification of viable cells; Preparation of
chemically competent E. coli cells; Transformation of E. coli; Small scale
isolation of recombinant plasmid from E. coli; Analysis of the recombinant
plasmid using Restriction Endonucleases; Agrobacterium-mediated plant
transformation & transient reporter gene expression assay; Analysis of a
transgene by PCR. Texts: 1.
H. S. Chawla, Laboratory Manual for
Plant Biotechnology, Oxford & IBH Publishing Co., New Delhi, 2003 2. S. J. Karcher, Molecular Biology: A Project Approach, Academic Press, 2001. References: 1.
J. Sambrook, D. W. Russell and J. Sambrook, Molecular Cloning, A laboratory Manual, Cold Spring Harbor
Laboratory, USA, 1999. 2.
J. G. Chirikjian, Biotechnology: Theory
and Techniques (Genetic Engineering, Mutagenesis and Separation Technology),
Jones & Bartlett Publishers, U.K., 1995. 3.
H. Jones and John M. Walker, Plant Gene
Transfer and Expression Protocols: Methods in Molecular Biology, 49, Humana Press, 1996. 4.
J. G. Chirikjian, Biotechnology: Theory
and Techniques (Plant Biotechnology, Animal Cell Culture and Immunobiotechnology),
Jones & Bartlett Publishers, U.K., 1996. 5. D. M. Glover and B. D Hames, DNA Cloning II, IRL Press, 1995. |
BT 310 Bioinformatics
and Computational Biology Laboratory (0-0-6-6) Molecular databases and their organization; Analysis of
Restriction sites in a known DNA fragment; Design of a specific PCR primer
for an amplicon; Homology search algorithm; Multiple sequence alignment and
phylogenetic analysis; Gene identification strategies; Identification of
structural and functional motifs; Visualization and analysis of protein
structure; Homology Modeling of protein. Texts: 1.
B. Bryan, Bioinformatics computing: the
complete practical guide to bioinformatics for lifescientists,
Prentice Hall, 2000. 2. S. Misener and S. A. Krawetz, Bioinformatics: methods and protocols,
Humana Press, 2000. References:
1.
D.
Baxevanis and B.F. F. Ouellette, Bioinformatics:
a practical guide to the analysis of genes and proteins, 2nd Ed., John Wiley & Sons, 2001. 2.
S. A. Krawetz and D. D. Womble, Introduction to bioinformatics: a theoretical and practical approach,
Humana Press, 2003. 3.
D. Higgins and W. Taylor (ed.), Bioinformatics:
sequence, structure and databanks-a practical Approach, Oxford, 2000. |
BT
302 Biochemical
Engineering (3-1-0-8) Unique features of the field, definitions and
concepts, introduction of organisms as mass and energy consumers, metabolic
stoichiometry and energetics, kinetics of substrate utilization, product
formation and biomass production in cell cultures, Transport phenomena in
bioprocess systems: gas-liquid mass transfer in cellular systems,
determination of oxygen transfer rates, Design and analysis of biological
reactors: batch, continuous, fed-batch, fluidized bed reactor, packed bed
reactor, bubble column, trickle bed reactor, animal and plant cell reactor
technology, aeration and agitation, Instrumentation and control: physical,
chemical and biosensors, online sensors, computers and interfaces, scale up, applied enzyme catalysis;
media and air sterilization, product recovery, bioprocess economics. Texts:
1.
H. W. Blanch and D. S. Clark, Biochemical
Engineering, Marcel, Dekker Inc., 1996. 2. J.
E. Bailey and D. F. Ollis, Biochemical
Engineering Fundamentals, 2nd Ed., McGraw-Hill Inc., 1986. References:
1. P. A. Belter, E. L. Cussler
and W. S. Hu, Bioseparations:
Downstream Processing for Biotechnology, John Wiley & Sons,
1988. 2.
H. J. Rehm and G. Reed, Biotechnology-A multi- Volume
Comprehensive Treatise, Vol 3,
2nd Ed., VCH, 1993 3.
M. Moo-Young, Comprehensive
Biotechnology, Vol 2, Pergamon Press, 2004 4.
S. Aiba, A. E. Humphrey and N. Millis, Biochemical
Engineering, Prentice-Hall 1978 5. P.
F. Stanbury and A. Whitaker, Principles
of fermentation technology, Pergamon press, 1984 6.
H. C. Vogel and C. L. Tadaro, Fermentation and Biochemical Engineering
Handbook - Principles, Process Design, and Equipment, 2nd Ed.,
William Andrew Publishing/Noyes, 1997. |
BT 305 Industrial Microbiology (3-0-0-6) Pre-requisite: BT 202 or equivalent. Basis
and development of Industrial Fermentation Processes (Screening cultures,
media, inoculum, scale-up); Control
of microorganisms; Fermentation Processes; Microbial production of
Antibiotics, Alcohols, Enzymes, Organic acids, Amino acids, Vitamins,
Biopolymers; Microbial
polysaccharides; Bioplastics;
Biosurfactants; Bioinsecticides; Pigments and flavors with their applications; Microbial leaching; Bio-transformation; Bio-degradation;
Food production involving
microorganisms and their products; Fermentation involving genetically
engineered microbes; Industrial applications of extremophiles; Safety aspects
of industrial processes. Texts: 1. M.
A. Malden, Industrial Microbiology: An
Introduction, Blackwell Science, 2001. 2.
A. N. Glazer and H. Nikaido, Microbial
Biotechnology: Fundamentals of Applied Microbiology, W. H. Freeman &
Co, 1995. References: 1. L.
Demain, R. M. Atlas, G. Cohen, C. L. Hershberger, W. S. Hu, D. H. Sherman, R.
C. Wilson and J. H. David, Manual of
Industrial Microbiology and Biotechnology, 2nd Ed, ASM Press,
1999. 2.
H. J. Rehm and G. Reed, Biotechnology:
A Comprehensive Treatise, VCH publisher, 2001. |
BT 306 IPR,
Ethics and Bio-Safety (3-0-0-6) General overview of
Intellectual Properties; Patent and
utility models; Design and trademark; Trade secret and unfair competition;
New plant varieties and geographical indication; Copyright and related
rights; International intellectual property treaties; Patents
structure and classification;
Patenting procedures; Economic impact of the patent system and legal issues; Licensing and enforcing intellectual
property; Commercializing an invention; Case studies; Scope of intellectual
properties in India –
Biodiversity and Traditional knowledge; Biosafety- classification and description of
biosafety levels; Design of clean rooms and biosafety labs; Biosafety
regulations to protect nature; Growers and consumers interest and nation
interest; Potential risk from genetically modified organisms; Ethical issues
in research and case studies. Texts: 1. C.B. Raju, Intellectual
Property Rights, Serial Publication, New Delhi, 2006 2. B.A.Brody and H.T. Engelhardt (Jr.), Bioethics: Reading & Cases, Person Education, Inc., 2007 References: 1. P.N. Cheremisinoff, R. P. Ouellette and R.M. Bartholomew, Biotechnology
Applications and Research, Technomic Publishing Co., Inc., USA, 1985. 2. D. Balasubramaniam, C.F.A. Bryce, K. Dharmalingam, J. Green
and K. Jayaraman, Concepts in Biotechnology, University Press (Orient
Longman Ltd.), 2002. 3. D. Bourgagaize, T.R. Jewell and R. G. Buiser, Biotechnology:
Demystifying the Concepts, Wesley Longman, USA, 2000. |
BT
308 Animal
Cell Biotechnology ( 3-0-0-6) Pre-requisite: BT 208 or equivalent Animal
cell biotechnology: Animal cell and tissue engineering; Animal cell culture
techniques relevant to mRNA knockdown (e.g. antisense and ribozyme
technology); generation of immortalized cell lines. In vitro organogenesis;
Stem Cells; Differentiation of animal and human cells; Animal cloning;
Mechanisms of drug resistance and cell death; Basic developmental Biology;
Structure and organization of tissues; Cell Surface markers; FACS analysis;
Cell Migration: control of cell migration in tissue engineering;
Transplantation immunology. Texts:
1.
T. A. Brown, Gene Cloning and DNA
Analysis: An Introduction, Blackwell Science, 2001. 2.
T. A. Brown, Genomes, 2nd
Ed., BIOS Scientific Publishers, 2002. References: 1.
B. R. Glick and J. J. Pasternak, Molecular
Biotechnology: Principles and Applications of Recombinant DNA, 3rd
Ed., ASM Press, 2003. 2.
R. I. Freshney, Animal Cell
Culture: A Practical Approach,
2nd Ed., IRL Press, 1992. 3.
R. E. Spier, Encyclopedia of Cell
Technology, Vols 1 and 2, Wiley Biotechnology Encyclopedia, John Wiley
& Sons, 2000. 4.
A. Doyle, J. B. Griffiths and D. G. Newell, Cell and Tissue Culture Laboratory Procedures, John Wiley & Sons,
1998. |
BT 330 Biochemical
Engineering Laboratory (0-0-6-6) Theory, operation and handling of instruments to be used in
this Lab course; Determination of biomass concentration by dry weight method;
Determination of bacterial specific growth rate and substrate utilization
rate in a batch operated reactor; Study of substrate inhibition kinetics of
bacterial growth in shake flask culture; Production and purification of an
enzyme from a microbial source; Determination of deactivation kinetics of a
soluble enzyme; Determination of Kla. Texts: 1.
J. E. Bailey and D. F. Ollis, Biochemical
Engineering Fundamentals, 2nd Ed., McGraw-Hill Inc., 1986. 2. P. A. Belter, E. L. Cussler and W. S. Hu., Bioseparations: Downstream Processing for
Biotechnology, John Wiley & Sons, 1988. References: 1.
H. J. Rehm and G. Reed., Biotechnology
- A Multi-volume Comprehensive Treatise, Vol.3, 2nd Ed., VCH,
1993. 2. M. Moo-Young, Comprehensive Biotechnology, Vol 2, Pergamon Press, 2004. 3. P. F. Stanbury and A. Whitaker, Principles of fermentation technology,
Pergamon Press, 1984. 4.
S. Aiba, A. E. Humphrey and N. Millis, Biochemical
Engineering, Prentice-Hall 1978. |
BT 404 Bioseparation
Engineering (3-0-0-6) Downstream
processing of products: filtration, centrifugation, sedimentation, solvent
extraction, aqueous two phase system, sorption, precipitation, chromatography
; whole broth processing; Cell separation: disruption by presses,
homogenizers, milling, sonication, and non mechanical methods; preparative
electrophoresis; product recovery schemes for antibiotics, commercial enzymes
and organic acids. Texts:
1.
T. Nagamune, S. Katoh and T. Yonemoto, Bioseparation
Engineering, Elsevier Science Publication, 2002. 2. M.
R. Ladisch, Bioseparations Engineering:
Principles, Practice and Economics, Wiley-Inter Science, 2001. References: 1. R. G. Harrison, P. Todd,
S. Rudge and D. P. Petrides, Biosperations
Sceince and Engineering, Oxford University Press, 2003. 2. P. A. Belter, E. L.
Cussler and W. S. Hu, Bioseparations:
Downstream Processing for Biotechnology, John Wiley & Sons, 1988. |
BT 405 Environmental Biotechnology (3-0-0-6) Introduction
to environment; pollution and its control; pollution indicators; waste
management: domestic, industrial, solid and hazardous wastes; strain
improvement; biodiversity and its conservation; microbes for bioremediation
technology such as petroleum, hydrocarbon decontamination, radionuclei, toxic
metal, dyes and lignin removal and xenobiotics; phytoremediation; biomass for
removal and biosorption of heavy metal and other inorganic ions; removal of
volatile organic compounds from waste gas. Texts:
1. B.
Ritmann and P. L. McCarty, Environmental
Biotechnology: Principle & Applications, 2nd Ed., McGraw
Hill Science, 2000. 2. G.
M. Evans and J. C. Furlong, Environmental
Biotechnology: Theory and Applications, Wiley Publishers, 2002. References: 1. H. S. Peavy, D. R. Rowe and G. Tchobanoglous, Environmental Engineering, McGraw-Hill
Inc., 1985. 2. J. S. Devinny, M. A. Deshusses and T. S. Webster, Biofiltration for Air Pollution Control,
CRC Press, 1998. 3. H. J. Rehm and G. Reed, Biotechnology – A Multi-volume Comprehensive Treatise, Vol. 11, 2nd Ed., VCH
Publishers Inc., 1993. |
BT 401 Frontiers
in Biotechnology (3-0-0-6) Introduction
to microfluidics and microfabrication, micropatterning and lithographic
techniques, applications of microfluidic systems in biology; Biosensors and
biofuel cells; Mechanisms, therapeutic and computational aspects of cancer,
AIDS and neurodegenerative disorders; Systems biology; Modern analytical
techniques in biotechnology. Texts: 1. J.Cooper, and T. Cass, Biosensors,
2nd Ed.,
Oxford University Press, 2004. 2. L. Pecorino, Molecular Biology of Cancer: Mechanisms, Targets, and Therapeutics,
2nd Edn., OUP, 2008. References: 1. J.B. Park and J.D. Bronzino, Biomaterials:
Principles and Applications. CRC Press. 2002. 2. T.S. Hin (ed.), Engineering Materials
for Biomedical Applications. World Scientific. 2004. 3. V. C. Yang and T. T. Ngo (eds.), Biosensors and their applications,
Kluwer Academic/Plenum Publishers, Moscow, 2000. 4. T. Finkel and J. S. Gutkind (eds.), Signal transduction and human disease,
Wiley Interscience, 2003. |
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