B. Tech Biosciences and Bioengineering

BT 306        Bio-separation Engineering        3-0-2-8

Syllabus: Overview: Broad categories of bioproducts, essential stages in downstream processing, basic unit operations in downstream processing; Solid-liquid separation: sedimentation, centrifugation, filtration, cell disruption, flocculation; Product isolation: extraction, adsorption, membrane-based separation; Product purification: precipitation, chromatography; Product polishing: crystallization, drying; Case studies: illustrative examples pertaining to downstream processing of bioproducts, biopharmaceuticals and recombinant products.

Texts:

1. P. A. Belter, E. L. Cussler and W. S. Hu, Bioseparations: Downstream Processing for Biotechnology, 1^st Edition, John Wiley & Sons Inc., 1988.

2. M. R. Ladisch, Bioseparations Engineering: Principles, Practice, and Economics, 1^st Edition, John Wiley & Sons Inc., 2001.

3. R. G. Harrison, P. Todd, S. R. Rudge, and D. P. Petrides, Bioseparations Science and Engineering, 1^st Edition, Oxford University Press, 2003.

References:

1. P. M. Doran, Bioprocess Engineering Principles, 2^nd Edition, Elsevier Ltd, 2013.

2. M. L. Shuler and F. Kargi, Bioprocess Engineering: Basic Concepts, 2^nd Edition, Prentice Hall, 2002.