Himanshu Singh
singh[AT*]iitg.ac.in
Research Areas:
Chemical Biology, Biophysical Chemistry, Molecular Biophysics, Structural Biology, and Biomolecular NMR Spectroscopy and Applications
Web page: https://www.hsingh-lab.com/
Our research focuses on understanding the link between the protein/nucleic acids conformational dynamics occurring on different lengths and time scales and their concerted role in biological function. In parallel to NMR method developments for biologically exciting problems, we use a combination of proton-detected solid- and solution-state NMR and MD simulations to elucidate the interplay between the functional active-site conformational changes, ligand binding events and allosteric mechanisms. Our long-term interest is to integrate protein dynamics knowledge with the structure and dynamics-guided evolution for a detailed understanding of the function and tailored interference with pharmacological targets.
Our research interests include the following topics:
1. Investigating the Protein Dynamics, Interactions and Allostery for Therapeutic Potential.
2. Protein Engineering for Nucleic Acids Modifications.
3. Investigating the Functions of Larger Biomolecules (100 kDa and beyond) by NMR: Allostery and Binding Networks.
4. Understanding the Behaviour of Proteins Linked to Brain Disorder.
5. Nuclear Magnetic Resonance (NMR) Method Developments (solution-, solid-state and high-pressure NMR) and Applications.
6. Developing Carbon Capture and Conversion (CCC) Technologies.
For more details: https://www.hsingh-lab.com/
PhD: Chemistry, Department of Chemical Sciences, Tata Institute of Fundamental research (TIFR) Mumbai, Colaba, Mumbai, India.
B.Tech: Industrial Biotechnology, Shri Mata Vaishno Devi University (SMVDU), Jammu, India.
2024-today: Assistant Professor, Department of Biosciences and Bioengineering, Indian Institute of Technology-Guwahati, Guwahati, Assam, India.
2022–2024: The Ramalingaswami Fellow, Assistant Professor, DBT, GOI, Department of Chemical Sciences, IISER Berhampur, Odisha, India.
2018–2022: Postdoctoral Research Associate, Department of Chemistry and Chemical Biology, Technical University of Dortmund, Dortmund, Germany.
2016–2018: Postdoctoral Research Associate, Department of Chemistry and Pharmacy, Ludwig Maximilians University (LMU), Munich, Germany.
Feb-Apr, 2016: Visiting Fellow, Department of Chemical Sciences, Tata Institute of Fundamental -Research, Mumbai and Hyderabad, India.
Mar 2015- Jun 2015: Visiting Scientist, Institute Pasteur-Paris, France.
Oct 2013-Nov 2013: Visiting Scientist, ETH Zurich, Switzerland.
May 2012-Jun 2012: Visiting Scientist, University of Florence, Italy.
01/2024-05/2024: Chemical Biology, CHM 617, BS-MS, IISER Berhampur, Odisha. (audience: BS-MS and PhD students).
08/2023-12/2023: Fundamentals of NMR Spectroscopy, BIO 417 (audience: BS-MS and PhD students).
08/2023-12/2023: Biophysical Chemistry, CHM 625, IISER Berhampur, Odisha. (audience: BS-MS and PhD students).
08/2023-12/2023: Graduate Seminar Course, CHM 803, IISER Berhampur, Odisha.
08/2023-12/2023: Biophysical Chemistry, CHM 625, IISER Berhampur, Odisha.
01/2023 to 05/2023: Chemical Biology, CHM 617, IISER Berhampur, Odisha. (audience: 4th-year BS-MS and PhD students).
08/2022 to 12/2022: Biophysical Chemistry, CHM 625, IISER Berhampur, Odisha. (audience: BS-MS and PhD students).
10/2019-01/2020, 12/2020-01/2021 and 11/2021-01/2022: Protein NMR Spectroscopy, TU Dortmund, Germany (audience: MS students).
09/2017-01/2018: NMR of Biomolecules, LMU, Munich, Germany (audience: MS and PhDs)
.Journals
1. AK Rout, S Gautam, VK Mishra, MD Bopardikar, B Dehury, and H. Singh " NMR insights into the beta-lactamase activity of UVI31+ from C. reinhardtii", J. Mag. Res. DOI., Vol.362, 107689, P.P - , (2024)
2. N Kumari and H. Singh "1H, 15N and 13C resonance assignments of S2A mutant of human carbonic anhydrase II", Biomol. NMR Assign. DOI.10.1007/s12104-024-10166-6, Vol., , P.P - , (2024)
3. H Singh, CK Das, BC Buchmuller, LV Schäfer, D Summerer, R Linser " Epigenetic CpG Duplex Marks Probed by an Evolved DNA Reader via a Well-Tempered Conformational Plasticity", Nucleic Acids Res. DOI.10.1093/nar/gkad134, Vol., , P.P - , (2023)
4. H Singh, CK Das, SK Vasa, K Grohe, LV Schäfer, R Linser "The active site of a prototypical “rigid” drug target is marked by extensive conformational dynamics", Angew. Chem., Int. Ed.. DOI., Vol.59, , P.P 22916-22921 , (2020)
5. H Singh, SK Vasa, H Jagra, P Rovo, C Paslack, CK Das, H Zipse, LV Schafer, and R Linser "Fast-microsecond dynamics of the protein-water network in the active site of human carbonic anhydrase II by solid-state NMR spectroscopy", J. Am. Chem. Soc.. DOI., Vol.49, 141, P.P 19276-19288 , (2019)
Conference Publication
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“Cost effective biosynthetic method for production of 13C-labeled biomolecules" H Singh, 2019, Patent registration no. 201911020231.
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