400 MHz FT-NMR spectrometer

Description:

Bruker AVANCE NEO NMR SPECT. 400 NanoBay system provides detailed structural information in various molecular systems. The system is dedicated for multi nuclei (1H, 13C, 19F and 31P) solution state NMR experiments. This system also records 2D-NMR such as COSY & NOESY spectra for solving the structure of complicated molecules. The Ascend 400 MHz superconducting magnet operates at 9.4 Tesla with 54 mm standard bore long hold. It has Electromagnetic Disturbance Suppression (EDS) that provides excellent screening efficiency against external magnetic field disturbances. It contains 24 accessed sample positions and also allows spinning of samples with 3-10 mm sample diameter for better spectral measurements.

Applications:

1. Analyses both functional and anatomical information, allowing relationships between the two to be determined.
2. Quantify motional properties of biomolecule and determine residual structure of unfolded proteins.
3. Aids in the study of living cells and provides detailed analysis of molecular solutions and body fluids.

Software used:

1. TopSpin 4.1.1 for recording and analysis of NMR spectrum.