Cloning and expression of GFP sequence/gene in the expression vector
1. Which method is commonly used to confirm the successful cloning of the GFP gene into theexpression vector?
2. Which method is commonly used for the amplification of the GFP gene during cloning?
3. What is the purpose of a selectable marker in an expression vector used for GFP cloning?
4. Which class of enzymes is commonly used for DNA ligation during GFP gene cloning?
5. Which region of the GFP gene is responsible for the protein's fluorescent properties?