Virtual Labs

a: Utilizes two labeled antibodies b: Involves a competitive reaction c: Antigen or antibody is immobilized directly d: Uses DNA technology for detection

2. What is the purpose of the substrate in an ELISA test?

a: To produce a colored or fluorescent signal b: To amplify the signal c: To detect the antigen or antibody d: To remove unwanted molecules

3. Which ELISA type is most suitable for quantitative analysis of antigens with high specificity?

a: Indirect ELISA b: Competitive ELISA c: Sandwich ELISA d: Open Sandwich ELISA

4. Which of the following is not a principle of ELISA (Enzyme-Linked Immunosorbent Assay)?

a: Specific antigen-antibody binding b: Use of an enzyme-conjugated secondary antibody c: Detection of a chromogenic or fluorescent signal d: Separation of unbound components by size exclusion chromatography

5. Arrange the following steps about ELISA in correct order.

incubate with antibody-enzyme complex that binds primary antibody
coat surface with antigen, block unoccupied sites with nonspecific protein
add substrate, formation of colored product indicates presence of specific antigen
incubate with primary antibody against specific antigen

a: i, iv, ii, iii b: i, iv, iii, ii c: ii, iv, iii, i d: ii, iv, i, iii

6. What is the key feature of competitive ELISA?

a: Two antibodies recognizing different epitopes are used b: Competition between labeled and sample antigen for binding sites c: Detection involves DNA technology d: Involves direct binding of antigens and antibodies

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