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Bioscience and Bioengineering Immunology and Microbiology Lab Experiments

Aim

Theory

Pretest

Procedure

Simulation

Posttest

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Aim

Theory

Pretest

Procedure

Simulation

Posttest

References

Feedback

Flow Cytometry of the Immune Cells

1. What is the purpose of flow cytometry?

a: To analyze the physical and chemical properties of cells and particles in a fluid sample b: To sequence DNA and RNA c: To visualize cells and tissues at the microscopic level d: To measure the mass of cells and particles in a sample

2. What is the purpose of sheath fluid?

a: To lubricate the cells b: To position the sample of cells into a single file for optical interrogation c: To increase the speed of the flow d: To increase volume of the cellular mixture

3. Which of the following is not a component of a flow cytometer?

a: Laser b: Fluidics system c: Microscope d: Detectors

4. What is forward scatter in flow cytometry?

a: The measurement of light scattered at an angle to the laser beam b: The measurement of light emitted by fluorescent molecules in the sample c: The measurement of light scattered in the same direction as the laser beam d: The measurement of light transmitted through the sample

5. Which of the following is not typically measured in flow cytometry?

a: Cell size b: Cell shape c: Cell surface markers d: Cell weight

6. What is fluorescence in flow cytometry?

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