Virtual Labs

Immunoprecipitation of protein from Cell Lysate using Antibody

1. Which of the following statements about immunoprecipitation (IP) is NOT true?

a: IP is a method that uses an antibody that binds to a particular protein to isolate and concentrate that protein from a sample. b: Because Protein A or Protein G beads attach to the Fc region of antibodies, making it easier to capture the antibody-protein complex, they are frequently utilized in IP. c: Because immunoprecipitation only enables the isolation of individual proteins, it cannot be used to investigate protein-protein interactions. d: The washing procedure, which aids in removing other components and proteins that are not particularly bound from the beads, is one of the most important procedures in IP.

2. Which of the following sums up immunoprecipitation's (IP) main goal the best?

a: To enhance particular DNA sequences b: To calculate the protein content of a sample c: Sorting proteins according to how electrically charged they are d: Using an antibody to separate a particular protein from a complicated combination

3. Of the following, which one is crucial to take into account when choosing an antibody for immunoprecipitation?

a: The target protein's isoelectric point b: The antibody's solubility in water-based solutions c: The antibody's affinity and specificity for the target protein d: The antibody's molecular weight