Virtual Labs

a: Layer the blood sample onto a density gradient medium b: Dilute collected blood with equal volume of PBS buffer c: Add an anticoagulant to the blood sample d: Centrifuge the blood sample at high speeds

2. What is the function of washing the isolated lymphocytes before staining with Leishman stain?

a: To remove residual Ficoll Hypaque solution and Platelets b: To lyse the red blood cells c: To prevent the lymphocytes from adhering to the slide d: To enhance the staining of the lymphocytes

3. What is the principle of Leishman staining?

a: It stains the cytoplasm of the cells b: It stains the nuclei of the cells c: It stains the cell membranes of the cells d: It stains the mitochondria of the cells

4. Which cell culture media is used to culture lymphocytes?

a: DMEM b: MacConkey Agar c: LB broth d: RPMI 1640

5. How do lymphocytes appear upon Leishman staining?

a: Pale pink cytoplasm with dark blue nuclei and orange-red granules b: Pink-Red c: Blue cytoplasm with dark purple nuclei d: Purple cytoplasm with dark blue nuclei

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