Virtual Labs

2D Gel Electrophoresis of the Egg Proteins

1. In a 2D gel where each spot represents a protein of a particular pI value and molecular weight, what will be your inference about two proteins whose positions are horizontal to each other in the gel?:

a: They have same molecular weight but different pI value. b: They have same pI value but different molecular weight. c: They have same pI value and same molecular weight. d: They have different pI values and different molecular weight.

2. If you perform electrophoresis of histones and myoglobins having pI 8.5 and 5.5 respectively, under denaturing conditions at pH 7.0, what will be the migration pattern of these proteins? :

a: histones will migrate towards the cathode (-); myoglobin will migrate towards the anode (+) b: myoglobins will migrate towards the cathode (-); histones will migrate towards the anode (+) c: both will migrate towards the cathode d: both will migrate towards the anode