2D Gel Electrophoresis of the Egg Proteins

1. In 2D gel electrophoresis, the second-dimension separation is performed at what angle relative to the first-dimension separation? :
2. Which of the following stains used for staining proteins in 2D gel electrophoresis is incompatible with mass spectrometry?:
3.In 2D gel electrophoresis, proteins are separated based on _____ in first dimension and based on _____ in second dimension.: