Virtual Labs

a: To enhance the binding of primary antibodies b: To reduce nonspecific binding of antibodies c: To increase the sensitivity of detection d: To stabilize the proteins on the membrane

a: Coomassie Blue staining b: Silver staining c: Ponceau S staining d: Ethidium bromide staining

a: Proteins are denatured for SDS Page analysis, but not for Native PAGE b: Proteins are detected using antibodies for SDS PAGE, an oligomers for Native Page c: Native PAGE involves stacking gel, while SDS-PAGE does not d: SDS PAGE separates proteins based on size and charge, while NATIVE-PAGE separates proteins based only on size

Implemention of Western Blot Analysis of the Cell Lysate using Antibody