Laboratory Protocols

B.Tech. Lab Protocols

BT 290: Biomolecular Analysis Laboratory

Experiment #	Title
1	Estimation of Protein
2	Estimation of Carbohydrate
3	Determination of Peroxide Value of lipid
4	Estimation of Ascorbic Acid
5	Estimation of Nucleic acid
6	Determination of melting temperature (T_m) of DNA
7	Partial purification of alkaline phosphate from potato and desalting of purified fraction
8	Electrophoresis of Protein (SDS-PAGE)
9	Activity staining and Isozyme analysis
10	Enzyme Kinetics
11	Protein Unfolding
12	Protein structure using structural coordinates procured from Protein Data Bank

BT 312: Analytical Biotechnology Laboratory

Experiment #	Title
1	Thin Layer Chromatography
2	Gel filtration Chromatography
3	Protein Extraction & Protein Estimation by Bradford reagent
4	Protein Unfolding
5	Basics of Microscopy & Immunolabelling & visualization of cells
6	Radial Immunodiffusion Assay Protocol
7	Determination of Concentration of Antibody by ELISA
8	Synthesis of Nano-particle and its Particle Size Analysis
9	Demonstration of Flow Cytometry with Experimental Sample
10	Demonstration of Real time PCR
11	Demonstration of Scanning Electron Microscopy using Experimental Sample
12	Demonstration of Confocal Microscopy
13	Demonstration of Atomic Force Microscopy with Experimental Sample

BT 330: Biochemical Engineering Laboratory

Experiment #	Title
1	Determination of biomass concentration by dry weight method
2	Determination of specific substrate utilization rate
3	Determination of specific growth rate
4	Growth kinetics at various substrate concentration
5	Deactivation kinetics of soluble enzyme
6	Batch operation in a bioreactor to study bacterial growth kinetics
7	Determination of K_La
8	Fed-batch operation to maintain exponential growth phase

M.Tech. Lab Protocols

BT 510: Analytical Biotechnology Laboratory

Experiment #	Title
1	Estimation of Carbohydrate
2	Estimation of DNA by Diphenylamine reagent
3	Analysis of DNA by Gel Electrophoresis
4	Determination of melting temperature (T_m) of DNA
5	Protein Extraction & Protein Estimation by Bradford reagent
6	Electrophoresis of Protein (SDS-PAGE)
7	Protein Unfolding
8	Biolistic Gun
9	HPLC
10	SEM
11	Confocal Microscopy
12	AFM

BT 520: Applied Biology and Bio-engineering Laboratory

Experiment #	Title
1	ASEPTIC CULTURE TRANSFER TECHNIQUE
2	BACTERIAL GROWTH CURVE and SPECIFIC GROWTH RATE
3	BIOINFORMATICS TOOLS FOR SEQUENCE ANALYSIS, DESIGN OF PRIMERS AND IDENTIFICATION OF RESTRICTION SITES
4	PREPARATION OF COMPETENT CELLS
5	TRANSFORMATION OF COMPETENT CELLS WITH PLASMID DNA
6	ISOLATION OF PLASMID DNA
7	RESTRICTION ANALYSIS OF PLASMID DNA
8	PROTEIN EXPRESSION ANALYSIS by SDS-PAGE
9	Agrobacterium tumefaciens-mediated plant transformation and transient reporter gene expression assay