# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
121 Interleukin-17A/F (IL-17A/F) (APTAF42) Protein Human Interleukin-17A/F 69 72.2 nM Binding Buffer (145 mM sodium chloride, 5.4 mM potassium chloride, 0.8 mM magnesium chloride, 1.8 mM calcium chloride, 20 mM Tris–HCl (pH 7.6) supplemented with 0.05% Tween20  59.42% 5'-GGGCUAGCUGAUCGUACCCAGUAGCGUGGCAUGGGGUGCCUAGUCGGGCGAUACUAACAGCUAACACCC-3' Adachi, H., et al. "Antagonistic RNA aptamer specific to a heterodimeric form of human interleukin-17A/F." Biochemie, 93 (2011): 1081-1088.
122 Keratinocyte Growth Factor (14F) Protein Keratinocyte Growth Factor (KGF) 69 0.3 pM Binding Buffer (DPBS, 0.5 mM MgCl2, 0.2% BSA, 0.02% sodium azide for PC-3, serum-free MEM growth medium, 0.1% BSA, 25 mM HEPES (pH 7.4), 1 ug/ml heparin for NIH3T3/KGFR) 56.52% 5'-GGGAGGACGAUGCGGUGGUCUCCCAAUUCUAAACUUUCUCCAUCGUAUCUGGGCAGACGACUCGCCCGA-3' Pagratis, N., et al. "Potent 2'-amino-, and 2'-fluoro-2'- deoxyribonucleotide RNA inhibitors of keratinocyte growth factor." Nature Biotechnology, 25(1997):68-73.
123 Clone 1 Protein Protein E (PE) 69 165 ug (~5 nmol) purified truncated His-tagged PE immobilized on Ni–NTA agarose (Qiagen) in 1.6 mL PBSM (PBS with 1 mM MgCl2) 63.77% 5?-CGACTGCAGAGCTTGCTACGTGATCAAATCGAGCTTTAACCCAACAGAGCATCCGTATCTATCCAAATGGGTACCGAGCTCGAATTCCC-3? Barfod, Anders et al. "In Vitro Selection of RNA Aptamers Directed Against Protein E: A Haemophilus influenzae Adhesin." Mol Biotech, 2014: 1-12
124 MCP-1 (ADR7)  Protein Monocyte chemoattractant protein-1 (mouse) 70 180 pM Selection Buffer (110 mM NaCl, 1 mM MgCl2, 20 mM HEPES (pH 7.0), 1 mM CaCl2, 5 mM KCl, 0.001% HSA, 0.05% Tween 20) 62.86% 5'-GGGAGGACGAUGCGGGAACUCACCGGGAAGAAGCCCGUUCCGUCACAGACAUGUUCCGCAUCGUCCUCCC-3' Rhodes, Andrew, et al. "The generation and characterisation of antagonist RNA aptamers to MCP-1." FEBS Letter, 506 (2001): 85-90.
125 OSM (ADR58) Protein Human Oncostatin M (OSM) 70 7 nM Wash Buffer: 0.1% I-block, 0.05% Tween 20 in SCHMK buffer (110 mM NaCl, 1 mM MgCl2, 20 mM HEPES (pH 7.0), 1 mM CaCl2, 5 mM KCl) 68.57% 5'-GGGAGGACGAUGCGGAUCGCCCUGAACCGGCCCAGCAGACUGCUGACGGCACGAUCCGCAUCGUCCUCCC-3' Rhodes, Andrew, et al. "The generation and characterization of antagonist RNA aptamers to human oncostatin M." Journal of Biological Chemistry, 275 (2000): 28555-28561.
126 Neurotensin receptor NTS-1 (P19) Protein Neurotensin receptor NTS-1 70 0.37 nM Binding Buffer (SHMCK buffer containing 0.1% (w/v) HSA, 0.2% (w/v) CHAPS, 0.04% (w/v) CHS, and 0.1% (w/v) LM) 60.00% 5'-GGGAGGACGAUGCGGACAGAUACGGAACUACAGAGGUCAAUUACGGUGGCCACGCAGACGACUCGCCCGA-3' Daniels et al. "Generation of RNA Aptamers to the G-Protein-Coupled Receptor for Neurotensin, NTS-1." Journal of Analytical Biochemistry, 305(2002): 214-226.
127 Cyclophilin B (M9-5) Cells Cyclophilin B (CypB) pancreatic cell line 71 1.5 nM Buffer F: 20 mM HEPES pH 7.5, 150 mM NaCl, 2mM CaCl2, and 3 mM MgCl2 69.01% 5'-GGGAGGACGAUGCGGGGACCUAUGCAGUAGCCAGUGUGGACUGGGCUGCCCCCCCCAGACGACUCGCCCGA-3' Ray, P. (2012). Comparing human pancreatic cell secretomes by in vitro aptamer selection identifies cyclophilin b as a candidate pancreatic cancer biomarker. J. Clin. Invest., 122(5), 1734-1741. doi: 10.1172/JC162385
128 Brain Penetrating Aptamer (A15) Tissue Brain capillary endothelia and parenchyma 71 N/A nM SELEX selection buffer: 10 mM HEPES (pH 7.4), 50 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, 0.01% bovine serum albumin. 59.15% 5'-GGGAGGACGAUGCGGCGUAUUGCGCGAGGAUUAUCCGCUCAUCGUUGUUGUUGUGCAGACGACUCGCCCGA-3' Cheng C, B Davidson, et al. (2013) In vivo SELEX for identification of brain-penetrating aptamers. Molecular Therapy - Nucleic Acids 2: e67.
129 von Willebrand Factor (VWF R9.3) Protein von Willebrand Factor (VWF) 76 1.2 nM Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA) 57.89% 5'-GGGAGGACGAUGCGGAUCGCGCUCUCCUGCUUAAGCAGCUAUCAAAUAGCCCACUCAGACGACUCGCUGAGGAUCC-3' S Oney, BA Sullenger, et al. (2007) "Antidote-Controlled Platelet Inhibition Targeting von Willebrand Factor with Aptamers." Oligonucleotides, 17:265-274.
130 Human Wild-type PAI-1 (WT-15) Protein Human Wild-type PAI-1 76 177 pM Binding Buffer E( 20 mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.05% CHAPS) 53.95% 5'-GGGAGGACGAUGCGGAUCAACUCACCGUAGGUCUAGUGAGAACUUCAAGUCUACUCAGACGACUCGCUGAGGAUCC-3' Blake et al. "Antimetastatic Potential of PAI-1-Specific RNA Aptamers." Oligonucleotides, 19(2009): 117-128.