# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
451 D-Tryptophan (MF-10) Small Organic D-Tryptophan 153 18 nM Column Buffer (4 mM Mg2+, 250 mM NaCl, 50 mM Tris C1 (pH 7.6)) Binding Buffer is (250 mM NaCl, 4 mM MgCl2, 50 mM Tris (pH 7.6)) Elution Buffer (20 mM EDTA (pH 8.0)) 53.59% 5' AGUAAUACGACUCACUAUAGGGAGAAUUCCGACCAGAAGUUGGCGUUGGCAUGACGCGGGGAAUCGGGUGCAUCGAUGACUACUCCUGGGCCCACGUCUGUUGUUGACGUCACAGCUUGAUUUAGGAUAGCGCUUGGGCAGUCGUGCAGUGGA 3' Famulok and Szostak. "Stereospecific Recognition of Tryptophan Agarose by in Vitro Selected RNA." Journal of American Chemical Society, 114 (1992): 3990
452 Fibrinogen (85A) (T-Modified) Protein Fibrinogen 90 6.2 µM 300 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl at pH 7.6 Not mentioned in dB, calculate 5' CCTTCGTTGTCTGCCTTCGTAGCGGATCGAATTACGCGTTAACGGCAACCGATAACGGGACCGATTGCACACCCTTCAGAATTCGCACCA 3' Li M., Huanh Z., Du L., Altier C., Fang H., Wang B. 2008. Selecting Aptamers for a Glycoprotein through the Incorporation of the Boronic Acid Moiety. Journal of American Chemical SOciety 138(38): 12636-12638.
453 Lup an 1 allergen ?-conglutin (SGQ) Protein ?-conglutin 11 0.58 nM Binding Buffer: 10 mM phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2, pH 7.4 81.82% 5' GGTGGGGGTGG 3' P Polo. Selection, characterisation and analytical application of DNA aptamer against the anaphylactic toxic allergen, ?-conglutin, Lup an 1. (Doctoral Thesis). Retrieved from Tesis Doctorals en Xarxa
454 Thrombin (15mer) Protein Thrombin 15 84 nM Selection Buffer: (20 mM Tris-acetate pH 7.4, 40mM NaCl, 5mM KCl, 1mM CaCl2, 1mM MgCl2) 60.00% 5' GGTTGGTGTGGTTGG 3' (A) Bock, Louis, et al. "Selection of single-stranded DNA molecules that bind and inhibit human thrombin." Nature 355, (1992): 564-566. (B) S Uehara et al. 30 Poly(dA)-Tailed Thrombin DNA Aptamer to Increase DNase-Resistance and Clotting Inhibitory Activity. Bull. Chem. Soc. Jpn. 81(2008): 1485ƒ??1491
455 Lead (Pb) binding aptamer Other Lead (Pb) 15 1 nM pH 7.0, 20 mM NaNO3, 8 mM Tris nitrate, 100 ?M NaCN, 25 °C 60.00% 5' GGTTGGTGTGGTTGG 3' B-F Ye et al. "Colorimetric photonic hydrogel aptasensor for the screening of heavy metal ions." Nanoscale 4(2012): 5998-6003.
456 HIV-1 TAR RNA Hairpin Loop Small Organic HIV-1 TAR RNA Hairpin Loop 19 50 nM R buffer (20 mM HEPES (pH 7.4), 140 mM potassium acetate, 20 mM sodium acetate, 3 mM magnesium acetate) 57.89% 5' CCCTAGTTAGCCATCTCCC 3' Sekkai et al. "In Vitro Selection of DNA Aptamers Against the HIV-1 TAR RNA Hairpin." Antisense and Nucleic Acid Drug Development, 12 (2002): 265-274.
457 Ampicillin (AMP17) Small Organic Ampicillin 19 13.4 nM Binding Buffer: 20mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM KCl, 5 mM MgCl2. 68.42% 5' GCGGGCGGTTGTATAGCGG 3' Song KM, Jeong E, Ban C, et al. (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem. 402:2153-2161
458 Ampicillin (AMP18) Small Organic Ampicillin 19 9.8 nM Binding Buffer: 20mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM KCl, 5 mM MgCl2. 42.11% 5' TTTAGTTGGGGTTCAGTTG 3' Song KM, Jeong E, Ban C, et al. (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem. 402:2153-2161.
459 Peroxidase DNAzyme Protein Hemin 20 29 nM Binding buffer: 20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO.  60.00% 5' ATTGGGAGGGATTGGGTGGG 3' Zhu L, et al. (2012) In vitro selection of highly efficient G-quadruplex-based DNAzymes. Anal. Chem. 84: 8383-8390.
460 Anti -?1-Adrenergic Receptor-Autoantibodies (Aptamer 110) Protein ?1-Adrenoceptor Autoantibodies(AABs) 21 n/a nM Buffer 1: Tris buffer pH 7.3, 0.1% Tween-20, DMEM, 0.1% Tween-20 Buffer 2: Phosphate buffer pH 7.5, 0.1% Tween-20 57.14% 5' ACAGTAACCGCGTGAGGTCGA 3' Haberland, A., et al. "Aptamer Neutralization of Beta1-Adrenoceptor Autoantibodies Isolated From Patients With Cardiomyopathies." Circulation Research, 109 (2011): 986-992.