# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
541 Beta-Estradiol Aptamer (BES.1) Small Organic Beta-Estradiol (Estrogen) 48 6 µM 1X Binding Buffer: 20 mm Tris (pH 7.4), 1 M NaCl, 10 mM MgCl2 58.33% 5' GGCTCTCGGGACGACATGGATTTTCCATCAACGAAGTGCGTCCGTCCC 3' Yang K, Pei R, Stefanovic D, Stojanovic M.N. (2011) Optimizing Cross-reactivity with Evolutionary Search for Sensors. J. Am. Chem. Soc.134:1642-1647.
542 hVap1_Stem Protein VEGF 48 5.6 nM APBB; 10 mM HEPES, pH 7.9, 125 mM NaCl, 25 mM KCl, and 1 mM MgCl2 56.25% Meng, Hsein-Wei, and John Pagano. "Discovering Aptamers by Cell-SELEX against Human Soluble Growth Factors Ectopically Expressed on Yeast Cell Surface." Plos-One 9.3 (2014): 1-12. Print.
543 L-Tyrosinamide (pe35) Small Organic L-Tyrosinamide 49 45 nM Binding Buffer (20 mM Tris (pH 7.6), 300 mM NaCl, 5 mM MgCl2) 55.10% 5' AATTCGCTAGCTGGAGCTTGGATTGATGTGGTGTGTGAGTGCGGTGCCC 3' Vianini et al. "In vitro selection of DNA aptamers that bind L-tyrosinamide." Bioorganic & Medicinal Chemistry, 9 (2001): 2543-2548.
544 DNA Blocker (Part of Lysozyme Aptasensor) Other Lysozyme 49 0.1 fM NEBuffer 2(10X): 50 mM NaCl, 10 mM Tris-HCL, 10 mM MgCl2 1 mM dithiothreitol, pH 7.9.  44.90% 5' ATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGCCCGTGA 3' Fu et al. "An ultrasensitive peroxidase DNAzyme-associated aptasensor that utilizes a target-triggered enzymatic signal amplification strategy." Chemical Communications, 47(2011): 9876-9878.
545 L-Selectin (LD201t1) Protein L-Selectin binding to sialyl Lewis X 49 3 nM Binding Buffer (20 mM Hepes, 111 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl, 8.9 mM NaOH final pH 8) and 1% globulin-free BSA. 46.94% 5' TAGCCAAGGTAACCAGTACAAGGTGCTAAAACGTAATGGCTTCGGCTTAC 3' Hicke, Brain, et al. "DNA Aptamers Block L-Selectin Function In Vivo Inhibition of Human Lymphocyte Traf?cking in SCID Mic." Journal of Clinical Investigation, 98 (1996): 2688-2692
546 L-Selectin Multivalent Aptamer Protein L-Selectin 49  0.75 nM  PBS 46.94% Chang EK, Eckert MA, Ali MM, Riazifar H, Pone EJ, Liu L, et al. (2015) Facile Supermolecular Aptamer Inhibitors of L-Selectin. PLoS ONE 10(3): e0123034. doi:10.1371/journal.pone.0123034
547 Histone H4 N-terminal tail (4.33) Protein H4 N-terminal tail 50 1.3 nM Low-salt conditions: 100 mM NaCl, 5 mM MgCl2, and 10 mM HEPES (pH 7.5).  60.00% 5' TGGTGGGGTTCCCGGGAGGGCGGCTACGGGTTCCGTAATCAGATTTGTGT 3' Yu et al. "Aptamers can Discriminate Alkaline Proteins with High Specificity." Chem. Biochem. , 12(2011): 2659-2666.
548 Digoxin (Truncated D1) Protein Digoxin 50 0.05 nM Selection Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween 20. 62.00% 5' AGCGAGGGCGGTGTCCAACAGCGGTTTTTTCACGAGGGAGGTTGGCGGTGG 3' Z Kiani et al. In vitro selection and characterization of deoxyribonucleic acid aptamers for digoxin. Analytica Chimica Acta 748(2012): 67-72.
549 Phorate (SS4-54) Small Organic Phorate 54 1.43 µM Selection buffer used in binding affinity assays: 300mM NaCl, 50 mM KCl, 10 mM MgCl2, 50 mM Tris/HCl, and pH 8.3 46.30% 5' AAGCTTTTTTGACTGACTGCAGCGATTCTTGATCGCCACGGTCTGGAAAAAGAG 3' Wang, L, et al. (2012). Selection of DNA aptamers that bind four organophosphorus pesticides. Biotechnol Lett, 2012(34), 869-874. doi: 10.1007/s10529-012-0850-6
550 Profenofos (SS4-54) Small Organic Profenofos 54 1.25 µM Selection buffer used in binding affinity assays: 300mM NaCl, 50 mM KCl, 10 mM MgCl2, 50 mM Tris/HCl, and pH 8.3 46.30% 5' AAGCTTTTTTGACTGACTGCAGCGATTCTTGATCGCCACGGTCTGGAAAAAGAG 3' Wang, L, et al. (2012). Selection of DNA aptamers that bind four organophosphorus pesticides. Biotechnol Lett, 2012(34), 869-874. doi: 10.1007/s10529-012-0850-6