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# | Aptamer Name | Aptamer Target (General) | Aptamer Target (Specific) | Length | Affinity | Buffer | GC Content | Sequence | Reference |
---|---|---|---|---|---|---|---|---|---|
481 | DNAzymeMB (Part of Lysozyme Aptasensor) | Protein | N/A | 28 | 0.1 fM | NEBuffer 2(10X): 50 mM NaCl, 10 mM Tris-HCL, 10 mM MgCl2 1 mM dithiothreitol, pH 7.9. | 57.14% | 5' AGGGACGGGCTAAGTAACTGTGGAGGGT 3' | Fu et al. "An ultrasensitive peroxidase DNAzyme-associated aptasensor that utilizes a target-triggered enzymatic signal amplification strategy." Chemical Communications, 47(2011): 9876-9878. |
482 | L-arginine (12-28 hairpin) | Small Organic | L-arginine | 28 | ~2.5 nM | Buffer for CD binding assay: 10 mM sodium phosphate buffer (pH 7.5) and 100 mM NaCl. | 60.71% | 5' GGGATCGAAACGTAGCGCCTTCGATCCC 3' | K Harada and A Frankel. (1995) Identification of two novel arginine binding DNAs. EMBO J, 14(23): 5798-5811. |
483 | Insulin-Linked Polymorphic Region Two-Repeat (ILPR2) | Peptide | Insulin | 28 | N/A nM | Tris buffer (20 mM Tris, 5mM KCL, pH 7.3) | 71.43% | 5' ACAGGGGTGTGGGGACAGGGGTGTGGGG 3' | Connor A C, McGown L B, et al. (2006) Insulin Capture by an Insulin-Linked Polymorphic Region G-Quadruplex DNA Oligonucleotide. J Am Chem Soc. 128: 4986-4991 |
484 | Sulforhodamine B (Clone 73 - min) | Small Organic | Sulforhodamine B | 29 | 190 nM | Selection Buffer: 0.1 M KCl, 5 mM MgCl2, 10 mM Na-HEPES (pH 7.4) | 82.76% | 5' CCGGCCAAGGGTGGGAGGGAGGGGGCCGG 3' | Wilson C, Szostak J. (1998) Isolation of a fluorophore-specific DNA aptamer with redox activity. Chem Biol. 5(11):609-617. |
485 | Thrombin (29mer) | Protein | Thrombin | 29 | 0.5 nM | 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2 | 62.07% | 5' AGTCCGTGGTAGGGCAGGTTGGGGTGACT 3' | Tasset, D. (1997). Oligonucleotide inhibitors of human thrombin that bind distinct epitopes. J. Mol. Biol., 1997(272), 688-698. |
486 | Kit-129 | Protein | CD117 | 29 | 12.21 nM | buffer composed of Dulbecco’s phosphate-buffered saline with calcium and magnesium and 5 mM MgCl2, 0.45% glucose and 100 mg/ml tRNA. 1 hr incubation with cells | 65.52% | 5' GCTCAACGCGGGACGGCTCTCCCATTGAC 3' | Development of an Efficient Targeted Cell-SELEX Procedure for DNA Aptamer Reagents Susanne Meyer et al. 2013 |
487 | StrepApt 2 | Protein | Streptavidin | 29 | 77 nM | NaCl (100 mM), MgCl2 (2 mM), KCl (5 mM), CaCl2 (1 mM), Tris-HCl (pH 7.6, 20 mM) | 68.97% | Ruigrok, Vincent J. B., et al. "Kinetic and Stoichiometric Characterisation of Streptavidin-Binding Aptamers." ChemBioChem 13 (2012): 829-836. Print. | |
488 | 2-PA | Protein | Anthrax Protective Antigen (PA) | 29 | 1.51 nM | 50 uL Human Serum, 5 mM HEPES | 58.62% | Byul Nim Oh, Sungeun Lee, Hye-Yeon Park, Jin-Ook Baeg, Moon-Young Yoon, and Jinheung Kim. 2011. Reference: Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties. Analyst, 2011, 136, 3384. DOI: 10.1039/c0an00978d | |
489 | Anti-FLAg M2 Antibody Aptamer (ABA) | Protein | Anti-FLAG M2 Antibody | 30 | 80 nM | 4% glycerol, 1 mM MgCL2, 1 mM DTT, 50 mM NaCl, 10 mM Tris-HCl, pH 7.5, r.t. | 40.00% | 5' TCGATTTCCTTAGTTGTCTTCCTTAGTGAG 3' | A Lakamp and M Ouellette. A ssDNA aptamer that blocks the function of the anti-FLAG M2 antibody. J. Nucleic Acids 2011: doi:10.4061/2011/720798 |
490 | HIV Reverse Transcriptase (PF1) | Protein | HIV RT | 30 | 80 nM | 50 mM Tris-HCl, pH 8, 1mM DTT, 80 mM KCl, 6 mM MgCl2 for ten minutes. | 46.67% | 5' AGGAAGGCTTTAGGTCTGAGATCTCGGAAT 3' | Y-T Lai and J DeStefano. DNA aptamers to human immunodeficiency virus reverse transcriptase selected by a primer-free SELEX method: characterization and comparison with other aptamers. Nucleic Acid Therapeutics 22(2012): 162-176. |