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# | Aptamer Name | Aptamer Target (General) | Aptamer Target (Specific) | Length | Affinity | Buffer | GC Content | Sequence | Reference |
---|---|---|---|---|---|---|---|---|---|
81 | Lup an 1 allergen, ß-Conglutin | Protein | ß-Conglutin | 93 | 360 nM | Binding Buffer: 10 mM Phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2, pH 7.4 | 53.76% | 5'-AGCTGACACAGCAGGTTGGTGGGGGTGGCTTCCAGTTGGGTTGACAATACGTAGGGACACGAAGTCCAACCACGAGTCGAGCAATCTCGAAAT-3’ | P Nadal et al. DNA aptamers against the Lup an 1 food allergen. PLoS One 7(2012):e35253 |
82 | Botulinum neurotoxin type A (BoNT A) Aptamer | Protein | BoNT A Light Chain | 93 | 87 nM | PBS pH 7.4, 300 mM NaCl, 3mM MgCl2, 5mM DTT, 1mg/ml BSA, 0.2 % Tween, 1mg/ml heparin | 51.61% | 5'-GGGAGGAGGAGAGATGTGAACTTGACAGCGUGCCUAGAAGUCCAAGCUUAAAUAACCACGCUCGACAAGCAGAAACTCTACACTGGACTGGCG -3' | Chang, T., Blank, M., Janardhanan, P., Singh, B., Mello, C., Blind, M., Cai, S. "In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxin." Biochemical and Biophysical Research Communications 396(2010): 854-860. |
83 | K19 | Protein | Siglec-5 | 95 | 12.37 nM | 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS) | 41.05% | 5?-GACGCTTACTCAGGTGTGACTCGAAGGGGTTGGGTGGGTTTATACAAATTAATTAATATTGTATGGTATATTTCGAAGGACGCAGATGAAGTCTC-3? | Yang, M. et al. "Developing aptamer probes for acute myelogenous leukemia detection and surface protein biomarker discovery." Journal of Hematology & Oncology 2014, 7:5. |
84 | Ethanolamine (14.3) | Small Organic | Ethanolamine | 96 | 6 nM | Binding Buffer (100 mM NaCl, 20 mM Tris/HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20). | 45.83% | 5'-ATACCAGCTTATTCAATTTGAGGCGGGTGGGTGGGTTGAATATGCTGATTACCCCATCGGAGAACGTTAAGGCGCTTCAGATAGTAAGTGCAATCT-3' | Mann, Doerthe, et al. "In vitro selection of DNA aptamers binding ethanolamine." Biochemical and Biophysical Research Communication, 338 (2005): 1928-1934. Tradmarked in patent, "New DNA aptamers specific for compunds that contain the ethylamino group, useful for diagnosis, prevention and treatment of ethanolaminosis, schizophrenia and neurodegeneration (Original document: DE 102005052275 (B4))" |
85 | Streptavidin (31) | Protein | Streptavidin | 96 | 56.7 nM | Binding buffer: 100 mM NaCl, 20 mM, Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20 | 42.71% | 5'-ATACCAGCTTATTCAATTCTATACTCCACTTTGCTATTTCTCGGTTCCTTCACGCGCCGATCGCAGGCTGATGAATTGAGATAGTAAGTGCAATCT-3' | Stoltenburg et al. "FluMag-SELEX as an advantageous method for DNA aptamer selection." Analytical and Bioanalytical Chemistry, 383 (2005): 83-91. |
86 | YPEN-1 Endothelial Cells (III.1) | Cells | YPEN-1 Endothelial Cells | 96 | N/A nM | Selection buffer: 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2, and 0.1% NaN3 with 1 ug/uL yeast tRNA. | 39.58% | 5'-ATACCAGCTTATTCAATTAGGCGGTGCATTGTGGTTGGTAGTATACATGAGGTTTGGTTGAGACTAGTCGCAAGATATAGATAGTAAGTGCAATCT-3' | Blank et al. "Systematic Evolution of a DNA Aptamer Binding to Rat Brain Tumor Microvessels." The Journal of Biological Chemistry, >b>276(2001): 16464-16468. |
87 | Anti Fumonsin B1 Toxin | Protein | Fumonsin Toxin B1 | 96 | 100 nM | 100 mM NaCl, 20 mM Tris, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6 incubate with target for 60 minutes | 33.33% | 5'-ATACCAGCTTATTCAATTAATCGCATTACCTTATACCAGCTTATTCAATTACGTCTGCACATACCAGCTTATTCAATTAGATAGTAAGTGCAATCT-3' | Mckeague et al. Screening and Initial Binding Assessment of Fumonisin B1 Aptamers: International Journal of Molecular Sciences |
88 | Mycobacterium tuberculosis (strain H37Rv) (aptamer NK2) | Protein | Virulent mycobacterium tuberculosis (H37Rv) | 97 | 31 nM | Binding buffer: 25 mM Tris-HCl, 50 mM KCl, 200 mM NaCl, 0.2 mM EDTA, 5.0% (v/v) glycerol, and 0.5 mM DTT. | 51.55% | 5'-GCGGGATCCTATGACGCATTGACCCACAACACACTACTGTCGCTCGGTTCGAACTTCGTGCGACTGTTCCCTATAGTGAGTCGTATTAGAATTCCGC-3’ | Fan et al. "Aptamer from whole-bacterium SELEX as new theraputic reagent against virulent Mycobacterium Tuberculosis." Biochem. Bioph. Res. Co., 357, (2007): 743-748. |
89 | Kanamycin A (#13_82) | Small Organic | Kanamycin A | 98 | 5.1 µM | Selection Buffer: 100 mM NaCL, 20 mM Tris-HCl pH 7.6, 2 mM MgCl2, 5 mM KCl, and 1 mM CaCl2 | 47.96% | 5'-ATACCAGCTTATTCAATTCAGGGCGGTATGAGGCTCGATCAAGGTCGGAGCGAGAATTTTTTCGCGGAGTCGGCTGGATCAGATAGTAAGTGCAATCT-3' | Stoltenburg R, Nikolaus N, Strehlitz B.(2012) Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics. J Anal Methods Chem. 2012:doi:10.1155/2012/415697 |
90 | Cholic Acid (9) | Small Organic | Cholic Acid | 100 | 50 pM | selection buffer (50 mM Tris–HCl, 300 mM NaCl, 30 mM KCl, 5 mM MgCl2, pH 7.6 | 41.00% | 5'-GTACCAGCTTATTCAATTACCGCGAAGAAGTGTCATTGTTTTGGAGATTCGAAGCGCTGTACACAGGTAATGAAGCCTTCTAAGATAGTATGTTCATCAG-3' | Kato, T., et al. "In vitro selection of DNA aptamers which bind to cholic acid." Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1493 (2000): |