# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
21 IgE (4.4.12)  Protein IgE 78 23 nM Binding buffer: PBS and 1 mM MgCl2 (pH 8.0) 47.44% 5'-AGCAGCACAGAGGTCAGATGTGAAACATAGCATATTTACTTATGTCGCCTTGCCGGTTCCTATGCGTGCTACCGTGAA-3’ Mendonsa, Shaun D. and Bowser, Michael T. "In Vitro Selection of High-Affinity DNA Ligands for Human IgE Using Capillary Electrophoresis." Analytical Chemistry, 76 (2004): 5387-5392.
22 TTF1 (A) Protein Thyroid Transcription Factor 1 78 3.36 nM Ni-NTA magnetic bead (Qiagen) were used with PBS-T (50 mM K2HPO4 pH 7.5, 150 mM NaCl, 0.05% Tween-20) 50.00% 5’-GGTATTGAGGGTCGCATCTCAAAAGGGGTGATTGCTTGCACAATGACAGGGTAGGACAGATGGCTCTAACTCTCCTCT-3’ Murphy et al. "An improved method for the in vitro evolution of aptamers and applications in protein detection and purification." Nucleic Acids Research, 31 (2003): e110.
23 Taq DNA polymerase (TQ21) Protein Taq DNA polymerase 78 36 pM  Binding Buffer (50 mM KCl, 2.5 mM MgCl2, 10 mM Tris-HCl (pH 8.3)) 47.44% 5'-TTCTCGGTTGGTCTCTGGCGGAGCGATCATCTCAGAGCATTCTTAGCGTTTTGTTCTTGTGTATGTTCGCTTTTCCC-3’ Sumedha and Dang. "Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR." Journal of Molecular Biology, 264 (1996): 268-78.
24 Lactobacillum acidophilius (hemag1P) Cells Lactobacillum acidophilius 78 13 nM Washed twice with binding buffer: 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2 47.44% 5'-AGCAGCACAGAGGTCAGATGTAGCCCTTCAACATAGTAATATCTCTGCATTCTGTGTGCCTATGCGTGCTACCGTGAA-3' Hamula et al. "Selection of Aptamers against Live Bacterial Cells." Analytical Chemistry, 80(2008): 7812-7819.
25 Enterotoxin B (APT SEB1) Protein Staphylococcus aureus Enterotoxin B 78 N/A nM Binding buffer: 10 mM phosphate buffer, 2.7 mM KCl, 140 mM NaCl, 0.05% Tween, pH 7.4, 47.44% 5'-GGTATTGAGGGTCGCATCCACTGGTCGTTGTTGTCTGTTGTCTGTTATGTTGTTTCGTGATGGCTCTAACTCTCCTCT-3' DeGrasse, J. (2012). A single-stranded DNA aptamer that selectively binds to Staphylococcus aureus enterotoxin b. PloS One, 7(3): e33410. doi: 10.1371/journal.pone.0033410
26 JH6 Cells NB4 AML cell line 78 2.77 nM 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS) 56.41% 5?-GACGCTTACTCAGGTGTGACTCGGTACGCCGCAAGACGAGTTGTGTATAAGCCGGCCGAAGGACGCAGATGAAGTCTC-3? Yang, M. et al. "Developing aptamer probes for acute myelogenous leukemia detection and surface protein biomarker discovery." Journal of Hematology & Oncology 2014, 7:5.
27 ACG03 Cells gastric cancer cell-line HGC-27 78 16.49 nM 4.5 g/l glucose, 5 mM MgCl2,2 mg/ml BSA and 0.2 mg/ml yeast tRNA dissolved in PBS buffer 60.26% 5'-ACGCTCGGATGCCACTACAGGGGGGTGGTCCTGAGGGTGGTGTGGTTGGTTTGGTTTCCTCATGGACGTGCTGGTGAC-3' Zhang, X. et al. "A cell-based single-stranded DNA aptamer specifically targets gastric cancer." The International Journal of Biochemistry & Cell Biology 2014, 46: 1-8.
28 AP-1 cJun/cJun Aptamer-19 Protein Activator Protein-1 Family cJun/cJun Homodimer 78 0.5 nM binding buffer (20 mM Tris (pH 7.9), 0.1 mM EDTA, 10% glycerol, 1 mM DTT, 150 mM KCl, 5 mM MgCl2, 0.04% NP-40) 53.85% 5’-GGGAGATCACTTACGGCACCGTATAGTCGTACATGAACGAGTGTGAGTGTTAAGCCTTTGGCGACAGGGCTCGGAACC-3’ Walters RD, McSwiggen DT, Goodrich JA, Kugel JF (2014) Selection and Characterization of a DNA Aptamer That Can Discriminate between cJun/cJun and cJun/cFos. PLoS ONE 9(6): e101015. doi:10.1371/journal.pone.0101015
29 GR-3 Protein glucagon receptor (GCGR) 81 52.7±5.1 nM 5mM MgCl2 and 4.5 g/L glucose in Dulbecco’s Phosphate Bufered Saline (D-PBS, pH=7.4), and 0.1mg/mL yeast tRNA and 1mg/L bovine serum albumin (BSA) to reduce nonspecifc binding. 46.91% 5’-ATCCAGAGTGACGCAGCAGATAAGTAGGTATCCGTTTGAAAAACTTTTCTGACCGTCCGACTATGGACACGGTGGCTTAGT-3’ Wang, Guodong, et al. "Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX". Nature: Scientific Reports (7): 7179. DOI:10.1038/s41598-017-05840-w
30 L-Selectin (LD201) Protein L-Selectin 79 1.8 nM Binding Buffer (20 mM Hepes (pH 7.5), 125 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl) plus 0.01% (wt/vol) human serum albumin. 46.84% 5'-CTACCTACGATCTGACTAGCCAAGGTAACCAGTACAAGGTGCTAAACGTAATGGCTTCGGCTTACTCTCATGTAGTTCC-3' Hicke, Brain, et al. "DNA Aptamers Block L-Selectin Function In Vivo Inhibition of Human Lymphocyte Traf?cking in SCID Mic." Journal of Clinical Investigation, 98 (1996): 2688-2692