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# | Aptamer Name | Aptamer Target (General) | Aptamer Target (Specific) | Length | Affinity | Buffer | GC Content | Sequence | Reference |
---|---|---|---|---|---|---|---|---|---|
11 | AraHH001 | Protein | Cardiac Troponin T (cTnT) | 40 | NA | Not available | 52.50% | 5'ACGTACCGACTTCGTATGCCAAC AGCCCTTTATCCACCTC3' | Xiaolin Yang, Ying Zhao, Lijuan Sun, Honglan Qi, Qiang Gao, Chengxiao Zhang. Sensors and Actuators B: Chemical. Electrogenerated chemiluminescence biosensor array for the detection of multiple AMI biomarkers (2017). https://doi.org/10.1016/j.snb.2017.10.108 |
12 | G5a3N.4 | Protein | HPV-16E7 protein | 75 | 1.9 µM | 1X PBS | 40.00% | 5’-TAATACGACTCACTATAGGGAGACCCAAGCCGATTTATTTTGTGCAGCTTTTGTTCCCTTTAGTGAGGGTTAATT-3’ | Julia D. Toscano-Garibay, Maria L. Benitez-Hess, and Luis M. Alvarez-Salas. 2011. Isolation and Characterization of an RNA Aptamer for the HPV-16 E7 Oncoprotein. Archives of Medical Research 42 (2011) 88-96 |
13 | Tetracycline | Small Organic | Tetracycline | 76 | 63.6 nM | Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween-20 | 68.42% | 5’-CGTACGGAATTCGCTAGCCCCCCGGCAGGCCACGGCTTGGGTTGGTCCCACTGCGCGTGGATCCGAGCTCCACGTG-3’ | Niazi JH, Lee SJ, Gu MB. (2008) Single-stranded DNA aptamers specific for antibiotics tetracyclines. Bioorg Med Chem. 16: 7245-7253 |
14 | RBP4 binding aptamer | Protein | Retinol-binding protein-4 (RBP4) | 76 | 201 nM | Binding Buffer: 100 mM NaCl, 25 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween-20, pH 7.6. | 47.37% | 5’-ATACCAGCTTATTCAATTACAGTAGTGAGGGGTCCGTCGTGGGGTAGTTGGGTCGTGGAGATAGTAAGTGCAATCT-3’ | SJ Lee et al. Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors Actuat. B-Chem.(2012): 243-248. |
15 | Nampt binding aptamer (NBA) | Protein | Nampt/Nicotinamide phosphoribosyltransferase | 75 | 72.52 nM | Binding Buffer: 100 mM NaCl, 25 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween-20, pH 7.6. | 47.37% | 5’-ATACCAGCTTATTCAATTGGGCGGTGGGGGGGGTAGTGGGTGTTATGGCGATCGTGGAGATAGTAAGTGCAATCT-3’ | Park, J.-W., Tatavarty, R., Kim, D. W., Jung, H.-T., & Gu, M. B. (2012). Immobilization-free screening of aptamers assisted by graphene oxide. Chem. Commun., 48(15), 2071–2073. doi:10.1039/c2cc16473f SJ Lee et al. Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors Actuat. B-Chem.(2012): 243-248 |
16 | U2 | Cells | U87-EGFRvIII | 76 | 3.37 nM | 0.45 g of glucose, 10 mg yeast tRNA, 5 mg salmon sperm DNA, 0.1 g BSA and 0.5 ml of 1 M MgCl2 in 100 ml of 1X phosphate-buffered saline [PBS] | 47.37% | 5’-ATCCAGAGTGACGCAGCATTTTGACGCTTTATCCTTTTCTTATGGCGGGATAGTTTCGTGGACACGGTGGCTTAGT-3’ | Wu, X. et al. "Cell-SELEX Aptamer for Highly Specific Radionuclide Molecular Imaging of Glioblastoma In Vivo." PLOS One 2014, 9:3. |
17 | U8 | Cells | U87-EGFRvIII | 76 | 4.35 nM | 0.45 g of glucose, 10 mg yeast tRNA, 5 mg salmon sperm DNA, 0.1 g BSA and 0.5 ml of 1 M MgCl2 in 100 ml of 1X phosphate-buffered saline [PBS] | 39.47% | 5’-ATCCAGAGTGACGCAGCATGAATCTTTTCTTTTGGTTTTGATATTTATAGTTGGTGAATGGACACGGTGGCTTAGT-3’ | "Cell-SELEX Aptamer for Highly Specific Radionuclide Molecular Imaging of Glioblastoma In Vivo." PLOS One 2014, 9:3. |
18 | U19 | Cells | U87-EGFRvIII | 76 | 16.7 nM | 0.45 g of glucose, 10 mg yeast tRNA, 5 mg salmon sperm DNA, 0.1 g BSA and 0.5 ml of 1 M MgCl2 in 100 ml of 1X phosphate-buffered saline [PBS] | 40.79% | 5’-ATCCAGAGTGACGCAGCATTTGTATCCTATTTTGTTTATGTAATTGTCGTTGATCATGTGGACACGGTGGCTTAGT-3’ | Wu, X. et al. "Cell-SELEX Aptamer for Highly Specific Radionuclide Molecular Imaging of Glioblastoma In Vivo." PLOS One 2014, 9:3. |
19 | U31 | Cells | U87-EGFRvIII | 76 | 8.10 nM | 0.45 g of glucose, 10 mg yeast tRNA, 5 mg salmon sperm DNA, 0.1 g BSA and 0.5 ml of 1 M MgCl2 in 100 ml of 1X phosphate-buffered saline [PBS] | 42.11% | 5’-ATCCAGAGTGACGCAGCATTTGTTTAATATGTTTTTTAATTCCCCTTGTGGTGTGTTGTGGACACGGTGGCTTAGT-3’ | Wu, X. et al. "Cell-SELEX Aptamer for Highly Specific Radionuclide Molecular Imaging of Glioblastoma In Vivo." PLOS One 2014, 9:3. |
20 | EpCAM EP166 | Protein | EpCAM | 77 | 5.48 nM | Binding buffer [4.5 g/L glucose, 5 mM MgCl2 and 10% FBS in Dulbecco’s PBS (Sigma)] Bovine Serum Albumin (1 mg/ml; Thermo Inc., USA) | 58.44% | 5'-CGCGGAAGCGTGCTGGGCCAACAGAGGGACAAACGGGGGAAGATTTGACGTCGACGACA CATAACCCAGAGGTCGAT-3' | Kim, J., W.; Kim, E., Y.; Kim, S., Y; Byun, S., K.; Lee, D.; Oh, K.; Kim, W., K.; Han, B., S.; Chi, S.; Lee, S., C.; Bae, K. Identification of DNA Aptamers toward Epithelial Cell Adhesion Molecule via Cell-SELEX. Molecules and Cells, 2014, 37(10), 742-746 ISSN: 0219-1032 |