# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
461 Ampicillin (AMP4) Small Organic Ampicillin 21 9.4 nM Binding Buffer: 20mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM KCl, 5 mM MgCl2. 71.43% 5' CACGGCATGGTGGGCGTCGTG 3' Song KM, Jeong E, Ban C, et al. (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem. 402:2153-2161.
462 Anti Aflatoxin M1 Protein Aflatoxin M1 21 0.0182 nM N/A 38.10% 5' ACTGCTAGAGATTTTCCACAT 3' Nguyen. Label Free detection of Aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor
463 Ky2 Aptamer Other Kanamycin 21 78.8 nM 20mM Tris-HCl, 50mM NaCl, 5mM KCl, 5mM MgCl2, pH 8.0 61.90% Kyung-Mi Song, Minseon Cho, Hunho Jo, Kyoungin Min, Sung Ho Jeon, Taisun Kim, Min Su Han, Ja Kang Ku, Changill Ban, "Gold nanoparticle-based colorimetric detection of kanamycin using a DNA aptamer". Anal. Biochem. 415 (2011) 175-181.
464 Mercury II (Hg2+) Other Mercury II (Hg2+) 22 40 nM 3-(N-morpholino)propanesulfonic acid (10mM, pH 7.0, NaCl (25mM), NaNO3 (500mM), and ethylenediamine (0.1 mM) 36.36% 5' TTCTTTCTTCCCCTTGTTTGTT 3' A. Ono and H. Togashi. "Highly selective oligonucleotide-based sensor for mercury(II) in aqueous solutions." Angew. Chem. Int. Ed. 43(2004):4300-4302.
465 Glycated human serum albumin (GHSA)  Protein GHSA 23 5.78 µM 1X PBS, 137mM [Na+], 2mM [Mg2+] 60.87% 5' TGCGGTTGTAGTACTCGTGGCCG 3' Apiwat et al. Graphene based aptasensor for glycated albuminin diabetes mellitus diagnosis and monitoring. Biosensors and Bioelectronics 82(2016)140ƒ??145.
466 a-Synuclein Oligomers (T-SO508) Protein a-synuclein oligomers 24 68 nM TBS-T buffer for ELONA assay: 10 mM Tris-HCl, 150 mM NaCl, 5 mM KCl, pH 7.4 with 0.05% Tween 20. 75.00% 5' GCCTGTGGTGTTGGGGCGGGTGCG 3' K Tsukakoshi. Selection of DNA aptamers that recognize a-synuclein oligomers using a competitive screening method. Anal Chem. 84(2012): 5542-5547
467 Antibac1 Peptide Peptidoglycan 55 415 µM PBS 1x 72.73% 5' TCGCGCGAGTCGTCTGGGGACAGGGAGTGCGCTGCTCCCCCCGCATCGTCCTCCC 3' Ferreira IM, de Souza Lacerda CM, de Faria LS, Corrêa CR, de Andrade AS. Selection of peptidoglycan-specific aptamers for bacterial cells identification. Appl Biochem Biotechnol. 2014 Dec;174(7):2548-56. doi: 10.1007/s12010-014-1206-6. Epub 2014 Sep 4. PMID: 25185503
468 Adenosine/ATP (DH25.42) Small Organic Adenosine and ATP 25 6 µM Column Buffer: 300 mM NaC1, 5 mM MgC12, 20 mM Tris (pH 7.6) 64.00% 5' CCTGGGGGAGTATTGCGGAGGAAGG 3' Huizenga and Szostak. "A DNA Aptamer That Binds Adenosine and ATP." Biochemistry, 34 (1995): 656-665.
469 PC12 Cells (Aptamer17a) Cells PC12 Cells 25 N/A nM Washed with selection buffer (5 mM MgCl in PBS) from and 1 mg/ml yeast tRNAfor 30 minutes 60.00% 5' GGTTGGATGTAAGGTTGGAGGGGGG 3' (A) Liu et al. "Dissection of the Functional Structure of Aptamer17, Which Specifically Recognizes Differential PC12 Cells." Nucleic Acid Therapeutics, 21(2011): 225-229. (B) Wang et al. "Single-stranded DNA Aptamers thatBbind Differentiated but not Parental Cells: Subtractive Systematic Evolution of Ligands by Exponential Enrichment." J. Biotechnol., 102(2003): 15-22
470 V7t1 targeting VEGF-121 Protein VEGF-121 25 1.1 nM  10% (v/v) human serum in TBSTE (TBSE containing 0.05% (v/v) of Tween-20). 72.00% 5' TGTGGGGGTGGACGGGCCGGGTAGA 3' Nonaka,Y., Sode, K., and Ikebukuro, K.(2010) "Screening and Improvement of an Anti-VEGF DNA Aptamer." Molecules, 15, 215-225.