# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
151 14-16 Protein Mouse P68 RNA Helicase (Ddx5) 80 30.8 nM For selection: 5 nmol of aptamer pool in 200 µl of PBS, injected systemically and allowed to circulate for 20 minutes For binding assay: 10 mM Tris-HCl (pH 7.0), 150 mM NaCl, 10% (v/v) glycerol, 2.5 µg/ml tRNA. 10 minute incubation at 37°C For affinity purification: 20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, 0.1% BSA, 0.1 µM tRNA at 4°C for 2 hours 65.00% 5'-GGGAGGACGAUGCGGCAGUGCCCAACCGGAACAACAACCACCGGCGGCUCCUGCUCAGACGACUCGCUGAGGAUCCGAGA-3' Mi et al. ƒ??In vivo selection of tumor-targeting RNA motifsƒ?. Nature Chemical Biology 1(2010): 22-24. DOI: 10.1038/NCHEMBIO.277
152 Integrin ?V subunit (aV-1) Protein Integrin aV subunit 81 2.7 nM Binding Buffer: PBS supplemented with 0.05% Tween-20 and 0.1% BSA.  58.02% 5'-GGGAGGACGAUGCGGCACACAUUCCCGUCCUCGAUACGUCUAGGCUUAGUGCCACUUGCUUAAUCCAGACGACUCGCCCGA-3' Gong, Q. et al,(2012). Selection strategy to generate aptamer pairs that bind to distinct sites on protein targets. Analytical Chemistry, 2012(84), 5365-5371.
153 Integrin ß3 subunit (ß3-1) Protein Integrin ß3 subunit 81 6.5 nM Binding Buffer: PBS supplemented with 0.05% Tween-20 and 0.1% BSA.  59.26% 5'-GGGAGGACGAUGCGGCCCAGAUUACUGUGGAGUGGUUGUCUGCGAAUCCUUCGUCCACCCAAUAGCAGACGACUCGCCCGA-3’ Gong, Q. et al,(2012). Selection strategy to generate aptamer pairs that bind to distinct sites on protein targets. Analytical Chemistry, 2012(84), 5365-5371.
154 CD4 (aptamer 12) Protein CD4 protein found on CD4+ T-Cells 86 0.5 nM Binding Buffer: PBS containing 1 mM MgCl2 51.16% 5'-GGGAGACAAGAAUAAACGCUCAAGUGACGUCCUGAUCGAUUGUGCAUUCGGUGUGACGAUCUUUCGACAGGAGGCUCACAACAGGC-3' K A Davis et al. "Staining of cell surface human CD4 with 2'-F-pyrimidine-containing RNA aptamers for flow cytometry." Nucleic Acids Res 26(1998):3915-3924.
155 CD4 (Aptamer 7)  Protein CD4 87 .5 nM Elution Buffer: (50 mM sodium phosphate, 150 mM NaCl, 2 M urea and 0.1% NaN3 (pH 7.2))  48.28% 5'-GGGAGACAAGAAUAAACGCUCAAUGUGUCGUCCUGGUACGAUUUUGGUAUAUAACCGUGGCUUUUCGACAGGAGGCUCACAACAGGC-3' Davis, K., et al. "Staining of cell surface human CD4 with 2'-F-pyrimidine-containing RNA aptamers for flow cytometry." Nucleic Acids Research, 26(1998): 3915-3924.
156 Rous Sarcoma Virus (A) Cells Rous Sarcoma Virus 87 20 nM Binding buffer: 2.5 mM MgCl2, 100 mM NaCl, 20 mM Tris-HCl (pH 7.4) 55.17% 5'-GGGAGCUCAGAAUAAACGCUCAAGGGUAGGGAUCGUUACCCCGACAUUUUAAUGGGCCGAUGUUUCGACAUGAGGCCCGGAUCCGGC-3' Pan et al. Isolation of virus-neutralizing RNAs from a large pool of random sequences." PNAS, 92(1995): 11509-11513.
157 RIG-I (CL9)  Protein RIG-I 92 37.2 nM 1X PBS with 1 mM MgCl2 50.00% 5'-GGGAGAGCGGAAGCGUGCUGGGCCACCAUCCGUAACUAGCUAAUACUUGUUAUCUUUUUAUUUUCAUAACCCAGAGGUCGAUGGAUCCCCCC-3' Hwang et al. "5'-Triphosphate-RNA-independent activation of RIG-1 via RNA aptamer with enhanced antiviral activity." Nucleic Acids Research 40(2011): 2724 - 2733
158 Anti-EGFR (E07) Protein hEGFR and mEGFR 93 2.4 nM 1X DPBS, 5mM MgCl2, pH 7.1-7.5 52.69% 5'-GGCGCUCCGACCUUAGUCUCUGUGCCGCUAUAAUGCACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCGGAACCGUGUAGCACAGCAGA-3' N. Li et al. "Inhibition of Cell Proliferation by an Anti-EGFR Aptamer." PLoS One 6(2011): e20299.
159 Anti-EGFR (E03) Protein mEGFR and hEGFR 93 29 nM 1X DPBS, 5mM MgCl2, pH 7.1-7.5 51.61% 5'-GGCGCUCCGACCUUAGUCUCUGUGCUAGUAUAUCGCACGGAUUUAAUCGCCGUAGAAAAGCAUGUCAAAGCCGGAACCGUGUAGCACAGCAGA-3' N. Li et al. "Inhibition of Cell Proliferation by an Anti-EGFR Aptamer." PLoS One 6(2011): e20299.
160 4-1BB ligand (M12-23) Protein 4-1BB ligand on CD8+ T Cells 96 40 nM Low Salt Binding Buffer: 20 mM Hepes, pH 7.4; 50 mM NaCl; 2 mM CaCl2 ;0.01% BSA. 58.33% 5'-GGGAGAGAGGAAGAGGGAUGGGCGACCGAACGUGCCCUUCAAAGCCGUUCACUAACCAGUGGCAUAACCCAGAGGUCGAUAGUACUGGAUCCCCCC-3' J. O. McNamara II et al.. Multivalent 4-1BB binding aptamers costimulate CD8+ T cells and inhibit tumor growth in mice. J. Clin. Invest. 118(2008):376-386.