# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
441 Yeast phenylalanine tRNA (B2) Small Organic Yeast phenylalanine tRNA 109 12 nM Selection Buffer (250 mM NaCl, 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 2 mM spermidine, 0.2 mM EDTA) 53.21% 5' GGGAAUUCCGCGUGUGCUACGUAUCUUCAGGCGGUAACUAACUGUGCUGAGUCUAAUCUUUGUGAGGGACGGUAACAUAUGGUUCCCGCGUGGUCCGUUCGGGAUCCUC 3' Scarabino, Daniela, et al. "tRNA prefers to kiss." EMBO Journal 18 (1999): 4571-4578
442 ERK 1/ ERK2 (Family II) Protein Extracellular Regulated Kinase 1 and 2 (ERK 1 and ERK2) 109 4.7 nM Buffer: 10 mM HEPES, pH 7.5; 10 mM MgCl2 45.87% 5' GGGAGAGCCAUACCUGACAAAGACGCUAGCGAAUUGGUUCCUCACUCAAAAGUAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUGACAGGUUACGCAUCCUGCAUCCUC 3' Seiwert, S., et al. "RNA aptamers as pathway-specific MAP kinase inhibitors." Chemistry & Biology, 7(2000): 833-834.
443 Biotin Small Organic Biotin 110 5.7 µM  Selection Buffer (0.1 M KCl, 5 mM MgCl2, and 10 mM Na-HEPES (pH 7.4)) 51.82% 5' GGAACACUAUCCGAUGGCACCGACCAUAGGCUCGGGUUGCCAGAGGUUCCACACUUUCAUCGAAAAGCCUAUGCUAGGCAAUGACAUGGACUCCUUGGUCAUUAGGAUCG 3' Wilson et al. "Functional Requirements for Specific Ligand Recognition by a Biotin-Binding RNA Pseudoknot." Journal of Biochemistry, 37(1998): 14410-14419.
444 Kanamycin B (K8) Small Organic Kanamycin B 111 180 nM Binding Buffer: 140 mM NaCl, 5 mM KCl, lmM MgCl2, and 20 mM HEPES at pH 7.5 52.25% 5' GGGAGCUCGGUACCGAAUUCUCGCCCUAUAGGGGUGUUGAGGGAAAUGUGUGCGACAAGGUGCGGUGGCCAGAACUUUUCGUUCUCAUCAAAAGCUUUGCAGAGGAUCCUU 3' Kwon et al., "In Vitro Selection of RNA against Kanamycin B." Molecules and Cells, 11 (2001): 303-311.
445 Protein Tyrosine Phosphatase (N71yc16)  Protein Protein tyrosine phosphatase (PTPase) 112 18 nM  Selection Buffer (20 mM Tris (pH 7.6), 150 mM NaCl, 5 mM MgCl2, 1 mM dithiothreitol; 100 µl final volume) 50.00% 5' GGGAGAUACCAGCUUAUUCAAUUCUGGCAAUGGGCUAUCCCAAGUGCUAGGCUUCAGGGAGCGAGGACCAGACGACGUACCUAACCCUAAGGUGAGAUAGUAAGUGCAAUCU 3' Bell, S., et al. "RNA Molecules That Bind to and Inhibit the Active Site of a Tyrosine Phosphatase." The Journal of Biological Chemistry, 273(1998):14309-14314.
446 Influenza B Hemagglutinin (A20) Protein Influenza B Hemagglutinin (HA) 114 720 pM Binding Buffer: 50 mM Tris-HCl, pH 7.5, 25 mM NaCl, 5 mM MgCl2. 64.04% 5' GGGAGCUCAGCCUUCACUGCACUCCGGCUGGUGGACGCGGUACGAGCAAUUUGUACCGGAUGGAUGUUCGGGCAGCGGUGUGGCAGGGAUGAGCGGCACCACGGUCGGAUCCAC 3' S. Gopinath et al. An efficient RNA aptamer against human influenza B virus hemagglutinin. J. Biochem. 139(2006): 837-846.
447 Theophylline + Flavin Mononucleotide (AR7) Protein Theophylline and FMN 117 N/A nM Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl2  51.28% 5' GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGUCUGGAUACCAUGCAUGAUGCACCUUGGCAGUCUUACAGAAGGACGUCGAAACGGUAGCGAGAGCUC 3' Seetharaman et al. "Immobilized RNA switches for the analysis of complex chemical and biological mixture." Nature Biotechnology, 19(2001):336-341.
448 Colicin E3 (F1-1) Protein Colicin E3 121 2 nM Binding Buffer (10 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 30 mM NH4Cl) 56.20% 5' GGGAGAAUUCCGACCAGAAGCUUACUGUCCUCCCUUCAGAGAGCGCGGGACCCUUAACUUGGGGCCCACGAACAGCUUCAGUUCCGUCUCGGCGUCAUAUGUGCGUCUACAUGGAUCCUCA 3' Hirao, I., et al. "In Vitro Selection of RNA Aptamers that Bind to Colicin E3 and Structurally Resemble the Decoding Site of 16S Ribosomal RNA." Biochemistry, 43 (2004): 3214-3221.
449 ppERK2/ERK2 Protein ppERK2/ERK2 123 4.7 nM Selection Buffer (10 mM HEPES (pH 7.5) and 10 mM MgCl2) 47.15% 5' GGGAGAGCCAUACCUGACAAAGACGCUAGCGAAUUGGUUCCUCACUCAAAAGUAGGGGAAAGCGUUAUUAAGAAACCAAAAUUUGACAGGUUACGCAUCCUGCAUCCUCCAGGUUACGCAUCC 3' Seiwert, S., et al. "RNA aptamers as pathway-specific MAP kinase inhibitors." Chemistry and Biology, 7 (2000): 833-843
450 Sialyl Lewis X (5) Other Sialyl Lewis X 133 0.085 nM Binding Buffer (150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2) 46.62% 5' CCGUAAUACGACUCACUAUAGGGGAGCUCGGUACCGAAUUCAAGGUACUCUGUGCUUGUCGAUGUGUAUUGAUGGCACUUUCGAGUCAACGAGUUGACAGAACAAGUAGUCAAGCUUUGCAGAGAGGAUCCUU 3' Jeong, Sunjoo, et al. "In Vitro Selection of the RNA Aptamer against the Sialyl Lewis X and Its Inhibition of the Cell Adhesion." Biochemical and Biophysical Research Communications, 281 (2001): 237ƒ??243