# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
61 Hepatocyte Growth Factor Receptor Protein HGF-R / c-Met 84 0.091 nM Binding Buffer: DPBS with 0.05% BSA 54.76% 5'-GGAGGGAAAAGTTATCAGGCTGGATGGTAGCTCGGTCGGGGTGGGTGGGTTGGCAAGTCTGATTAGTTTTGGAGTACTCGCTCC-3’ Boltz A, Plater B, Hock B, et al. (2011) Bi-specific Aptamers Mediating Tumour Cell Lysis. J Biol Chem. 286: 21896-21905
62 Non-small Cell Lung Cancer (S6) Cells Non-small Cell Lung Cancer 85 28.2 nM Wash Buffer (5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbecco’s PBS without calcium and magnesium) The binding buffer was prepared by adding 1 mg/mL bovine serum albumin (BSA) and 0.1 mg/mL yeast tRNA to washing buffer 58.82% 5'-ACGCTCGGATGCCACTACAGGTGGCCAGTCACTCAATTGGGTGTAGGGGTGGGGATTGTGGGTTGCTCATGGACGTGCTGGTGAC-3' Zhao et al. "Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells." Analyst 134(2009):1808-1814.
63 Ricin Toxin (C5) Small Organic Ricin Toxin 86 58 nM Running Buffer for Capillary Electrophoresis: 20 mM HEPES (pH 7.1), 40 mM NaCl, 5 mM KCl, 1 mM CaCl2, and 1 mM MgCl2. Incubated at room temperature. 56.98% 5'-ATAGGAGTCACGACGACCAGAACCGTAGGTTCGGGGCGGAGTGGTCCGGAAGGTGGCGTGGTATGTGCGTCTACCTCTTGACTAAT-3' J Tang et al. "The DNA aptamers that specifically recognize ricin toxin are selected by two in vitro selection method." Electrophoresis, 27 (2006): 1303-1311.
64 LPS (B2) Small Organic Lipopolysaccharide (LPS) 86 12 nM Selection buffer: (50 mM Tris-acetate, pH 8.2) 61.63% 5'-CTTCTGCCCGCCTCCTTCCTAGCCGGATCGCGCTGGCCAGATGATATAAAGGGTCAGCCCCCCAGGAGACGAGATAGGCGGACACT-3’ Kim, S. (2012). Harnessing aptamers for electrochemical detection of endotoxin. Analytical Biochemistry ,424(2012), 12-20. doi: 10.1016/j.ab.2012.02.016
65 MUC1 (MA3) Protein MUC1 86 38.3 nM Binding Buffer: Hanks buffer with 0.1 mg/ml salmon sperm DNA and 1 mg/ml BSA. 50.00% 5'-AACCGCCCAAATCCCTAAGAGTCGGACTGCAACCTATGCTATCGTTGATGTCTGTCCAAGCAACACAGACACACTACACACGCACA-3’ Y Hu et al. Novel MUC1 aptamer selectively delivers cytotoxic agent to cancer cells in vitro. PLoS ONE 7(2012): e31970.
66 HER-2 (HB5) Protein HER2 peptide 86 18.9 nM Hanks buffer with 0.1 mg/mL salmon sperm DNA and 1 mg/mL of BSA 43.02% 5'-AACCGCCCAAATCCCTAAGAGTCTGCACTTGTCATTTTGTATATGTATTTGGTTTTTGGCTCTCACAGACACACTACACACGCACA-3’ Z Liu et al. Novel HER2 aptamer selectively delivers cytotoxic drug to HER2-positive breast cancer cells in vitro. J Transl Med 10(2012): 148-157.
67 HAE N55 Cells Inflamed Human Aortic Endothelial Cells 86 82 nM Wash buffer: 5 mmol/L MgCl2, 4.5 g/L glucose, in Dulbecco PBS. Binding buffer: 0.1 % bovine serum albumin (BSA) 0.1 g/L yeast tRNA + Wash Buffer 34.88% 5’-ATACCAGCTTATTCAATTCCCAAATTGCCACCACTTACAGCATGATAACATACTACATCTTTTCATCAAGATAGTAAGTGCAATCT-3’ Ji, Kaili., Lim, Wee Siang., Li, Sam Fong Yau., Bhakoo, Kishore. (2013) A two-step stimulus-response cell-SELEX method to generate a DNA aptamer to recognize inflamed human aortic endothelial cells as a potential in vivo molecular probe for atherosclerosis plaque detection. Anal Bioanal Chem (2013) 405:6853-6861. DOI 10.1007//s00216-013-7155-z
68 V. parahemolyticus (A3P) Cells Vibrio parahemolyticus 87 16.88 nM Binding Buffer: 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2 41.38% 5'-ATAGGAGTCACGACGACCAGAATCTAAAAATGGGCAAAGAAACAGTGACTCGTTGAGATACTTATGTGCGTCTACCTCTTGACTAAT-3' Duan, N et al. Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticus. J. Agri. Food Chem., 60(2012): 4034-4038.
69 HPV-HF cell line (20) Cells nontumorigenic HF cell line 87 1.6 nM 1% BSA in PBS with salts 44.83% 5'-ATACCAGCTTATTCAATTGGGGAGGGAGACACAGTCATGGAGCAGTTATTAGGGTGTACCGGGTGTAGTAGATAGTAAGTGCAATCT-3' Graham JC, Zarbl H (2012) Use of Cell-SELEX to Generate DNA Aptamers as Molecular Probes of HPV-Associated Cervical Cancer Cells. PLoS ONE 7(4): e36103. doi:10.1371/journal.pone.0036103
70 Adipose Tissue (adipo-8) Cells Mature 3T3-L1 adipocytes (adipose tissue) 87 17.8 nM 4.5 mg/ml glucose and 5 mM MgCl2 in D-PBS with calcium chloride and magnesium chloride (Sigma), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml). pH 7.1-7.5. 4°C 56.32% 5'-ATGAGAAGCGTCGGTGTGGTTAAACACGGAACGAAGGTGCAGGAAGATTTGTCGATGCGGTGCCTGAGCGGGCTGGCAAGGCGCATA-3' J. Liu et. al. "Selection of aptamers specific for adipose tissue." PLoS One, 7(2012): e37789.