# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
71 Anti Anterior Gradient Homolog 2 Protein Anterior Gradient Homolog 2 (AGR2) 87 13.16 nM 200 mL, 1X PBS buffer containing 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4 and 2.0 mM KH2PO4, pH 7.4 62.07% 5'-TCTCGGACGCGTGTGGTCGGGTGGGAGTTGTGGGGGGGGGTGGGAGGGTTCTTTGTTTGATCTTTCTCGCTGCCTGGCCCTAGAGTG-3' Wu et al. Identification, Characterization and Application of a G-Quadruplex Structured DNA Aptamer against Cancer Biomarker Protein Anterior Gradient Homolog 2 (PLOSone)
72 CCRF-CEM (sgc8) Cells Acute lymphoblastic leukemia (CCRF-CEM) 88 0.8 nM Binding buffer: 0.1mg/ml yeast tRNA and 1 mg/ml BSA into wash buffer (4.5g/L glucose, 5 mM MgCl2 in Dulbecco's PBS with CaCl2 and MgCl2) 42.05% 5'-ATACCAGCTTATTCAATTAGTCACACTTAGAGTTCTAACTGCTGCGCCGCCGGGAAAATACTGTACGGTTAGATAGTAAGTGCAATCT-3' Shangguan et al. "Optimization and Modifications of Aptamers Selected from Live Cancer Cell Lines." Chem. Biochem., 8, (2007): 603-6
73 Staphylococcus aureus (SA23) Cells Staphylococcus aureus 88 61.5 nM Screening buffer: 0.1 mg/ml salmon sperm DNA (0.1 mg/ml tRNA, 1%BSA, 1 PBS, 0.05% (v/v) Tween-20 52.27% 5'-GCAATGGTACGGTACTTCCGGGCTGGCCAGATCAGACCCCGGATGATCATCCTTGTGAGAACCACAAAAGTGCACGCTACTTTGCTAA-3' Cao et al. "Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus." Nucleic Acids Research, 37(2009): 1-8
74 Anti Staphylococcus aureus (SA20) Cells Staphylococcus aureus (S.Aureus) 88 70.86 nM  0.1mg/ml salmonsperm DNA (0.1mg/ml tRNA, 1%BSA, 1PBS, 0.05% (v/v) Tween-20) Incubated with cells for 1 hour 53.41% 5?-GCAATGGTACGGTACTTCCGCGCCCTCTCACGTGGCACTCAGAGTGCCGGAAGTTCTGCGTTATCAAAAGTGCACGCTACTTTGCTAA-3? Cao et al. "Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus." Nucleic Acids Research, 37(2009): 1-8
75 Anti Influenza NS1 protein Protein NS1 88 19.91 nM 50 mM Tris/Cl (pH 8.0), 150 mM NaCl, 1.5 mM MgCl2, 2 mM DTT and 1% (w/v) BSA 67.05% 5'-GCAATGGTACGGTACTTCCGCGGTCCGGGGTGGGTGGGTGGTGGGGGGTGCGGGGGGGCGGCCGCAAAAGTGCACGCTACTTTGCTAA-3’ Hye-Min Woo. Single-stranded DNA aptamer that specifically binds to the influenza Virus NS1 protein suppresses interferon antagonism
76 NS-1 Aptamer Protein Influenza A virus (IAV) non-stuctural protein (NS1) 88 18.91 nM 50 mM Tris/Cl (pH 8.0), 150 mM NaCl, 1.5 mM MgCl2, 2 mM DTT and 1% (w/v) BSA 67.05%
77 Nanobioprobe mediated DNA aptamer Other Trinitrotoluene (TNT) 88 100 nM  10 mM Tris/HCl buffer, pH 7.6, 150 mM NaCl, 5 mM MgCl2 46.59%
78 HPV-HF cell line (14) Cells HPV-nontumorigenic HF cell line 89 7.1 nM 1% BSA in PBS with salts 48.31% 5'-ATACCAGCTTATTCAATTGGGCGGGGAGTAGGGAGAGGGGTTTCCATCGGCGACAGAGGAGTTATGTGTGTAGATAGTAAGTGCAATCT-3’ Graham JC, Zarbl H (2012) Use of Cell-SELEX to Generate DNA Aptamers as Molecular Probes of HPV-Associated Cervical Cancer Cells. PLoS ONE 7(4): e36103. doi:10.1371/journal.pone.0036103
79 JH19 Cells NB4 AML cell line 89 7.57 nM 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml yeast tRNA and 1 mg/ml Bovine Serum Albumin (BSA) into Phosphate buffered saline (PBS) 56.18% 5?-GACGCTTACTCAGGTGTGACTCGAGGTGTGACTCGATCTGTGGGGGTTGGGGGGTGGTTTTTCGGAACGAAGGACGCAGATGAAGTCTC-3? Yang, M. et al. "Developing aptamer probes for acute myelogenous leukemia detection and surface protein biomarker discovery." Journal of Hematology & Oncology 2014, 7:5.
80 Fibrinogen (85A/Ap90) Protein Fibrinogen 90 63.8 nM Binding Buffer: 300 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl at pH 7.6 55.56% 5'-CCTTCGTTGTCTGCCTTCGTAGGACCGCAGACATCGACGCAGGGAAATTCCGCAAGTCCAGCCAAATGCCACCCTTCAGAATTCGCACCA-3' Minyong Li, Na Lin, Zhen Huang, Lupei Du, Craig Altier, Hao Fang and Binghe Wang. "Selecting Aptamers for a Glycoprotein through the Incorporation of the Boronic Acid Moiety." J. Am. Chem. Soc., 2008, 130 (38), pp 12636ƒ??12638.