# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
221 EphA2 Receptor (SWL peptide) Tissue EphA2 Receptor 12 678 nM Blocked with 0.5% BSA in TBS and washed with binding buffer (TBS, 1 mM CaCl2, 0.1% Tween 20). NH2-SWLAYPGAVSYR-COOCOOH Koolpe et al. "An Ephrin Mimetic Peptide That Selectively Targets the EphA2 Receptor." J. Biol. Chem., 49(2002): 46974 ƒ??4697
222 VEGF receptor KDR/Flk-1 (K237) Protein VEGF receptor KDR/Flk-1 12 N/A nM Washing Buffer: 0.9% NaCl, 0.01 M phosphate buffer, pH 6.2, blocked with 0.3% BSA, 25 mM HEPES (pH 7.6) for 3 h. NH2-HTMYYHHYQHHL-COOCOOH Hetian et al. "A novel peptide isolated from a phage display library inhibits tumor growth and metastasis by blocking the binding of vascular endothelial growth factor to its kinase domain receptor." J Biol Chem., 277(2002): 43137-42.
223 VEGF receptor Flt-1 (F56) Cells VEGF receptor Flt-1 12 N/A nM Washing Buffer (150 mM NaCl and 10 mM phosphate buffer, pH 6.2) and then blocked in 3% BSA (25 mM HEPES, pH 7.6) for 3 hours at room temperature. NH2-WHSDMEWWYLLG-COOCOOH An et al. "Suppression of Tumor Growth and Metastasis by a VEGFR-1 Antagonizing Peptide Identified from a Phage Display Library." Int. J. Cancer, 111(2004): 165ƒ??173.
224 Concanavalin A Lectin Protein Concanavalin A Lectin 12 46 µM Con A Buffer (CAB: 50 mM NaCl/20 mM Mops (pH 6.8)/2 mM MgCl2/2 mM CaCl2/0.2 mM EDTA)], room temperature NH2-DVFYPYPYASGS-COOCOOH Oldenburg et al. " Peptide ligands for a sugar-binding protein isolated from a random peptide library." PNAS, 89(1992):5393-5397.
225 E-Selectin Protein E-Selectin 12 4 nM Assay Buffer (PBS, 0.1% BSA, 424 µM Ca(NO3)2, 406 µM MgSO4, 0.02% Tween 20), 4 °C. NH2-DITWDQLWDLMK-COOCOOH Martens et al. "Peptides Which Bind to E-selectin and Block Neutrophil Adhesion." The Journal of Biological Chemistry, 270 (1995):21129-21136
226 Gastrin-Releasing Peptide (GRP-1) Protein Gastrin-Releasing Peptide Receptor (GRPR) aka BB2 Receptor) 12 N/A nM Peptide binding assays: Phosphate-buffered saline (PBS) with 1% fetal calf serum (FCS).
227 ErbB2 Tyrosine Kinase Receptor (Ph-12-1) Protein ErbB2 Tyrosine Kinase Receptor 12 N/A nM N/A 5'-WNLPWYYSVSPT-3' Shadidi and Sioud. "Identification of Novel Peptides for the Specific Delivery of Therapeutics into Cancer Cells." The FASEB Journal, 2, (2003): 256-8
228 Human Colorectal Cancer WiDr Cell Line (HEW-K16) Cells Human Colorectal Cancer (WiDr Cell Line) 13 N/A nM  107 cells/mL in PBS and 1.0% BSA with 1.5x1011 phages/mL NH2-HEWSYLAPYPWFK-COOCOOH Rasmussen et al. "Tumor cell-targeting by phage-displayed peptides." Cancer Gene Therapy, 9(2002): 606-612
229 Mouse Lung Vasculature  Tissue Mouse Lung Vasculature 13 N/A nM  In vivo in mice. NH2-CGFECVRQCPERC-COOCOOH D Rajotte et al. " Molecular Heterogeneity of the Vascular Endothelium Revealed by In Vivo Phage Display." Journal of Clinical Investigations, 102(1998): 430-437.
230 Mouse Skin Vasculature Tissue Mouse hypodermis 13 N/A nM Perfusion and washing was performed with DME. NH2-CVALCREACGEGC-COOCOOH D Rajotte et al. " Molecular Heterogeneity of the Vascular Endothelium Revealed by In Vivo Phage Display." Journal of Clinical Investigations, 102(1998): 430-437.