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# | Aptamer Name | Aptamer Target (General) | Aptamer Target (Specific) | Length | Affinity | Buffer | GC Content | Sequence | Reference |
---|---|---|---|---|---|---|---|---|---|
311 | Ricin A-chain (RTA) Aptamer | Protein | Ricin A-chain (RTA) | 26 | 7.4 nM | 1X PBS, 5 mM MgCl2 | 69.23% | 5' GGCGAACAGGGGACGAGCAAGACGCC 3' | (A) (1) Bogomolova, A., Aldissi, M. ??Real-time and label-free analyte detection in a flow-through mode using immobilized fluorescent aptamer/quantum dots molecular switches.? Biosensors and Bioelecgtronics, 66 (2015): 290-296 (B) Hesselberth, J., et al. In vitro Selection of RNA Molecules That Inhibit the Activity of Ricin A-chain. |
312 | GRK2 | Protein | G protein-coupled receptor kinase 2 | 28 | 1.2 nM | 20 mM Tris (pH 7.0), 50 mM NaCl, 5 mM MgCl2,2 mM DTT, and 0.1% Lubrol | 57.14% | 5' GGCAGACCAUACGGGAGAGAAACUUGCC 3' | Tesmer et al., Molecular Mechanism for Inhibition of G Protein-Coupled Receptor Kinase 2 by a Selective RNA Aptamer, Structure (2012) |
313 | 1-PA | Protein | Anthrax Protective Antigen (PA | 29 | 8.53 nM | 50 uL Human Serum, 5 mM HEPES | 48.28% | Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties. Analyst, 2011 | |
314 | cAMP | Small Organic | cAMP | 31 | 10 µM | 20 mM Tris-HCl (pH 7.5 at 23 °C), 450 mM NaCl, 100 mM KCl, 10 mM MgCl2, 1 mM MnCl2, and 5 mM CaCl2 | 51.61% | 5' GGAAGAGAUGGCGACUAAAACGACUUGUCGC 3' | Kozumi and Breaker. "Molecular Recognition of cAMP by an RNA Aptamer." Biochemistry, 39 (2000) |
315 | Xanthine | Small Organic | Xanthine | 32 | 3.3 nM | 20 mM TrisHCl (pH 7.5), 0.3 M NaCl and 5 mM MgCl2 | 59.38% | 5' GGCACGUGUAUUACCCUAGUGGUCGACGUGCC 3' | Kiga, D., et al. "An RNA aptamer to the xanthine/guanine base with a distinctive mode of purine recognition." Nucleic Acids Research, 26 (1998) |
316 | L-Isoleucine | Small Organic | L-Isoleucine | 32 | 200 nM | Selection Buffer (10 mM HEPES (pH 7.0), 300 mM NaCl, and 7.5 mM MgCl2 (buffer B) and 0.1 mM each of ZnCl2, CaCl2, and MnCl2 and 0.01 mM CuCl) Reaction Buffer (50 mM sodium borate (pH 8.0), 42 mM KCl, 7.5 mM MgCl2, and 0.4 mM ZnCl2) | 56.25% | 5' GGUCUUACGUCGUUCGCGACUAUUGGGAGACC 3' | Majerfeld and Yarus. "Isoleucine:RNA sites with associated coding sequence." RNA, 4 (1998): 471-478. |
317 | Moloney Murine Leukemia Virus | Protein | Moloney Murine Leukemia Virus | 33 | 6.9 nM | 50 mM Tris-HC1 (pH 7.7), 200 mM potassium acetate, and 10 mM DTT | 57.58% | 5' UUACCACGCGCUCUUAACUGCUAGCGCCAUGGC 3' | Selection of High-Affinity RNA Ligands to Reverse Transcriptase: Inhibition of cDNA Synthesis and RNase H Activity." Journal of Biochemistry, 33(1994) |
318 | Basic Fibroblast Growth Factor | Protein | Basic Fibroblast Growth Factor | 34 | 190 pM | Selection Buffer (1X PBS = 10.1 mM Na2HPO4/1.8 mMKH2PO4/137 mM NaCl/2.7 mM KCl, pH 7.4) | 52.94% | 5' GGUGAAGGCAACGUAUAGGCAAGCACACUUCACC 3' | Jellinek, D., et al. "High-affinity RNA ligands to basic fibroblast growth factor inhibit receptor binding." Proceedings of the National Academy of Sciences of the United States of America, 90(1993): |
319 | GL21.T | Protein | Axl tyrosine kinase receptor | 34 | 12 nM | Dulbecco's Modified Eagle's Medium (DMEM), 10% FBS, 2mM L-glutamine | 50.00% | 5' AUGAUCAAUCGCCUCAAUUCGACAGGAGGCUCAC 3' | Laura Cerchia, Carla L Esposito, Simona Camorani, Anna Rienzo, Loredana Stasio, Luigi Insabato, Andrea Affuso and Vittorio de Franciscis,"Targeting Axl With an High-affinity Inhibitory Aptamer". Molecular Therapy vol.20 no.12, 2291-2303, Dec2012. |
320 | SP6 RNA polymerase | Protein | SP6 RNA Polymerase | 35 | 9.5 nM | Binding Buffer (50mM Tris-HCl, pH 7.6, 1 M NaCl, 10mM 2-mercaptoethanol, 10% glycerol) at 4°C overnight at -20°C until use | 40.00% | 5' UUGCUUGGAAUGCGUUAUAGUCUCUUAGGUGUGUA 3' | Mori, Y. (2012). Inhibitory RNA aptamer against sp6 RNA polymerase. Biochemical and Biophysical Resarch Communications, 420 (2012), 440-443. |