# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
51 Human Norovirus SMV-19 Other Human Norovirus 81 191 nM 500 ml of binding buffer [consisting of phosphate buffered saline (pH 7.1) supplemented with 100 mg/L CaCl2, 100 mg/L MgCl2 and 0.05% Tween 20] at Room Temperature 46.91% 5’-AGTATACGTATTACCTGCAGCCACCAGTGTGTTGAGGTTTGAGCACACTGATAGAGTGTCACGATATCTCGGAGATCTTGC-3’ Escudero-Abarca BI, Suh SH, Moore MD, Dwivedi HP, Jaykus L-A (2014) Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains. PLoS ONE 9(9): e106805. doi:10.1371/journal.pone.0106805
52 Human Norovirus SMV-21 Other Human Norovirus 81 101 nM 500 ml of binding buffer [consisting of phosphate buffered saline (pH 7.1) supplemented with 100 mg/L CaCl2, 100 mg/L MgCl2 and 0.05% Tween 20] at Room Temperature 43.21% 5’-AGTATACGTATTACCTGCAGCCCATGTTTTGTAGGTGTAATAGGTCATGTTAGGGTTTCTGCGATATCTCGGAGATCTTGC-3’ Escudero-Abarca BI, Suh SH, Moore MD, Dwivedi HP, Jaykus L-A (2014) Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains. PLoS ONE 9(9): e106805. doi:10.1371/journal.pone.0106805
53 Human Norovirus SMV-25 Other Human Norovirus 81 232 nM 500 ml of binding buffer [consisting of phosphate buffered saline (pH 7.1) supplemented with 100 mg/L CaCl2, 100 mg/L MgCl2 and 0.05% Tween 20] at Room Temperature 43.21% 5’-AGTATACGTATTACCTGCAGCCATCTGTGTGAAGACTATATGGCGCTCACATATTTCTTTCCGATATCTCGGAGATCTTGC-3’ Escudero-Abarca BI, Suh SH, Moore MD, Dwivedi HP, Jaykus L-A (2014) Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains. PLoS ONE 9(9): e106805. doi:10.1371/journal.pone.0106805
54 Aptamer 25 Other Human norovirus (SMV-VLP) 81 232 nM Phosphate buffered saline (pH 7.1) supplemented with 100 mg/L CaCl2, 100 mg/L MgCl2 and 0.05% Tween 20 43.21% 5’-AGTATACGTATTACCTGCAGCCATCTGTGTGAAGACTATATGGCGCTCACATATTTCTTTCCGATATCTCGGAGATCTTGC-3’ Escudero-Abarca BI, Suh SH, Moore MD, Dwivedi HP, Jaykus L-A (2014) Selection, Characterization and Application of Nucleic Acid Aptamers for the Capture and Detection of Human Norovirus Strains. PLoS ONE 9(9): e106805. doi:10.1371/journal.pone.0106805
55 Bovine Catalase (CAT 1) Protein Bovine Catalase 82 237 nM CE Selection Buffer (1X TGK): 25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3). 60.98% 5'-CTTCTGCCCGCCTCCTTCCGACCTAGCAGTGGACATGTGGCAGGGTGAAGTGGCATCGTCGGAGACGAGATAGGCGGACACT-3’ Ashley, J. et al. Selection of bovine catalase aptamers using non-selex. Electrophoresis 33(2012):2783-2789
56 Hepatitis C Virus RdRp Protein Hepatitis C Virus RdRp 83 1.3 nM Binding buffer: HEPES-buffered saline containing 1 mM CaCl2, 2.7 mM MgCl2, and 0.005% surfactant P-20 (HBS-CKM) 53.01% 5'-GGGAGACAAGAATAAACGCTCAAGGGCGTGGTGGGTGGGGTACTAATAATGTGCGTTTGTTCGACAGGAGGCTCACAACAGGC-3' Jones et al. "High-Affinity Aptamers to Subtype 3a Hepatitis C Virus Polymerase Display Genotypic Specificity." Antimicrobial Agents and Chemotherapy, 50 (2006): 3019-3027
57 Vaccinia virus–infected A549 cells Cells Vaccinia virus (WR Strain)–infected A549 cells 83 2.7 nM Binding Buffer: Dulbecco PBS, 4.5g/L glucose, 5 mmol/L MgCl2, 0.1 g/L yeast tRNA, 100 mL/L FBS, and 1 g/L BSA. 45.78% 5'-ATCGTCTGCTCCGTCCAATATGATGACACCTGCATAATTTATAGTGAGTCTTGATTCACGCTGCATTTGGTGTGAGGTCGTGC-3’ Tang et al. "Generating Aptamers for Recognition of Virus-Infected Cells." Clin. Chem., 55(2009): 813-822.
58 Anti Glioblastoma Cells (GBM128) Cells Glioblastoma Cell 83 20 nM 25 µM glucose 4 µM yeast tRNA 15 µM BSA 5 mM MgCl2 in PBS (pH = 7.4) 1 hour incubation with cells 49.40% 5'-GAATTCAGTCGGACAGCGACGGTGGGAGCCCCAAATAATTCTTGCGATTATTAGTGTAAGCGGATGGACGAATATCGTCTCCC-3’ Kang et al. Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity (PLOSone
59 GBM128 Cells Glioblastoma 83 20 nM  The washing buffer (WB) used contained 4.5 g/L glucose, and 5 mM MgCl2 in PBS (pH = 7.4). The binding buffer used during selection was prepared by adding yeast tRNA (0.1 mg/mL) and BSA (1 mg/mL) to washing buffer to reduce background binding. 49.40% 5'-GAATTCAGTCGGACAGCGACGGTGGGAGCCCCAAATAATTCTTGCGATTATTAGTGTAAGCGGATGGACGAATATCGTCTCCC-3’ Kang et al. Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity (PLOSone
60 Aptamer TY04 Cells Multiple myeloma cells (MM.1S 83 3.89 µM yeast tRNA (0.1mg/ml; Sigma) and BSA (1 mg/ml; Sigma) into the wash buffer 45.78% 5'-ATCGTCTGCTCCGTCCAATATATCAAAGGCGAATTTTGTCAAGGTGTTAAACGATAGTCCCTACCTTTGGTGTGAGGTCGTGC-3’ Dai, H. et al. "Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest." Tumor Biology 2014: 1-8