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# | Aptamer Name | Aptamer Target (General) | Aptamer Target (Specific) | Length | Affinity | Buffer | GC Content | Sequence | Reference |
---|---|---|---|---|---|---|---|---|---|
581 | Thrombin (15mer-29-mer dimer with 20T linker) | Protein | Thrombin | 64 | 0.35 nM | Binding Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM KCl, pH 8.0. | 42.19% | 5' GGTTGGTGTGGTTGGTTTTTTTTTTTTTTTTTTTTAGTCCGTGGTAGGGCAGGTTGGGGTGACT 3' | H. Hasegawa et al. Improvement of aptamer affinity by dimerization. Sensors 8(2008): 1090-1098 |
582 | Mouse Transferrin Receptor (GS24) | Protein | Mouse Transferrin Receptor (mTfR) | 64 | N/A nM | PBS with 1 mM MgCl2 | 51.56% | 5' GAATTCCGCGTGTGCACACGGTCACAGTTAGTATCGCTACGTTCTTTGGTAGTCCGTTCGGGAT 3' | E F Neufeld et al. Aptamer-based endocytosis of a lysosomal enzyme. Proc. Natl. Acad. Sci. USA 105(2008): 15908-15913 |
583 | Ochratoxin A (H12) | Small Organic | Ochratoxin A (OTA) | 66 | 96 nM | Binding Buffer: PBS 1X, 1 mM MgCl2, 0.01% Tween 20, pH 7.4. | 63.64% | 5' GGGAGGACGAAGCGGAACCGGGTGTGGGTGCCTTGATCCAGGGAGTCTCAGAAGACACGCCCGACA 3' | L Barthelmebs et al. Enzyme-linked aptamer assays (ELAAs), based on a competition format for a rapid and sensitive detection of ochratoxin A in wine. Food Control 22(2011): 737-743 |
584 | Hodgkin Lymphoma aptamer | Cells | HDLM2 | 66 | 10 nM | Washing buffer for selection: Dulbecco’s PBS with 4.5 g/L glucose and 5 mM MgCl2s. Binding buffer to reduce nonspecific background binding: 1 mg/mL BSA and 0.1 mg/mL t-RNA | 46.97% | Parekh, P. et al. "Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma." Sensors 2013, 13: 14543-14557. | |
585 | PS1 | Cells | HDLM2 | 66 | 10 nM | 4.5 g/L glucose, 5 mM MgCl2, 1 mg/mL BSA and 0.1 mg/mL t-RNA | 46.97% | Parekh, P. et al. "Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma." Sensors 2013, 13: 14543-14557. | |
586 | Acute myeloid leukemia cells (KH1C12) | Cells | Acute myeloid leukemia cells (HL60) | 68 | 4.5 nM | Binding Buffer (4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml tRNA and 1mg/ml BSA, dissolved in Dulbecco's PBS with MgCl2 and CaCl2) containing 20% FBS. | 47.06% | 5' ATCCAGAGTGACGCAGCATGCCCTAGTTACTACTACTCTTTTTAGCAAACTGGACACGGTGGCTTAGT 3' | Sefah, Kwame, et al. "Molecular recognition of acute myeloid leukemia using aptamers." Leukemia, 23 (2009):235-244 |
587 | Tenascin-C (GBI-10) | Protein | Tenascin-C (TNC) | 69 | 150 nM | Binding Buffer (10 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, 0.1% (wt/vol) BSA, 0.1 % (vol/vol) Triton X-100, 0.1% (vol/vol) Tween 20) | 55.07% | 5' GGCTGTTGTGAGCCTCCTCCCAGAGGGAAGACTTTAGGTTCGGTTCACGTCCCGCTTATTCTTACTCCC 3' | Dion, Daniels, et al. "A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment." PNAS 100(2003): 15416-15421. |
588 | Crude Extracellular Mixture of E. coli - trans | Protein | Crude Extracellular Matrix of E. coli | 70 | N/A nM | Selection Buffer (1 mM HEPES (pH 7.4), 150 mM NaCl, 15 mM MgCl2, and 0.01% Tween 20), 60 min @ r.t. | 51.43% | 5' GATGTGCGTTGTCGAGACCTGCGACCGGAACACTACACTGTGTGGGGATGGATTTCTTTACAGTTGTGTG 3' | Aguirre et al. "A Sensitive DNA Enzyme-Based Fluorescent Assay for Bacterial Detection" Biomolecules, 3 (2013): 563-577. |
589 | Small-Cell Lung Cancer (HCH07) | Cells | Small-cell lung cancer (SCLC) cell-surface molecular markers | 71 | 38 nM | Washing Buffer (4.5 g/L Glucose, 5mM MgCl2, and 1 mg/mL BSA in Dulbecco's PBS (pH 7.3)) Binding Buffer (wash buffer and 0.1 mg/mL yeast tRNA) | 49.30% | 5' TACCAGTGCGATGCTCAGGCCGATGTCAACTTTTTCTAACTCACTGGTTTTGCCTGACGCATTCGGTTGAC 3' | Chen, Hui, et al. "Molecular Recognition of Small-Cell Lung Cancer Cells Using Aptamers." CHEMMEDCHEM, 3 (2008): 991-1001. |
590 | Tumour Marker MUC1 (S1.6) | Peptide | Tumour Marker MUC | 72 | 0.2131 nM | Binding Buffer (0.2 M NaHCO3 and 0.5 M NaCl (pH 7.4)) | 48.61% | 5' GGGAGACAAGAATAAACGCTCAAGCAACAGGGTATCCAAAGGATCAAATTCGACAGGAGGCTCACAACAGGC 3' | Ferreira, C., et al. "DNA Aptamers That Bi nd to MUC1 Tumour Marker: Design and Characterization of MUC1-Binding Single-Stranded DNA Aptamers." Tumor Biology, 27(2006): 289ƒ??301. |