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# | Aptamer Name | Aptamer Target (General) | Aptamer Target (Specific) | Length | Affinity | Buffer | GC Content | Sequence | Reference |
---|---|---|---|---|---|---|---|---|---|
391 | L-Valine (ID# 153) | Small Organic | L-Valine | 83 | 12 mM | Selection Buffer (25 mM HEPES, 200 mM NaCl, 6 mM MgCl2, 1 mM EDTA (pH 7.3)) | 55.42% | 5' GGGAGCUCAGAAUAAACGCUCAAAUCCGUGGACAGGGCGUAAGCGCCUUCGACAUGAGACACGGAUCCUGCGACGAAUUCAGC 3' | Majerfeld and Yarus. "An RNA Pocket for an Aliphatic Hyrdrophobe." Nature Structural Biology, 1(1994): 287-292. |
392 | Anti-NF-kB p65 (R1) (ID# 387) | Protein | NF-kB p65 | 83 | 11 nM | Selection buffer: 10mM HEPES at pH 7.5, 100mM NaCl, 1mM DTT | 48.19% | 5' GAAGCUUACAAGAAGGACAGCACGAAUAAAACCUGCGUAAAUCCGCCCCAUUUGUGUAAGGGUAGUGGGUCGAAUUCCGCUCA 3' | Wurster, S. E, and Maher, L. J. "Selection and characterization of anti-NF-kB p65 aptamers." RNA, 14(2008): 1037-1047. |
393 | E. Coli Release Factor 1 (Clone II-1) (ID# 122) | Small Organic | E. Coli Release Factor 1 | 85 | 30 nM | Elution Buffer (250 mM imidazole) | 52.94% | 5' GGACCGAGAAGUUACCCUGUAAUCUUAGGAUGAAUCGCAUGCUCUAGCGACCUUUUCGGCUUCGGCGUACGCACAUCGCAGCAAC 3' | Sando, et al. "In vitro selection of RNA aptamer against Escherichia coli release factor 1." Bioorganic & Medicinal Chemistry Letters, 17(2007): 1216-1220. |
394 | Trypanosoma brucei (2-16) (ID# 145) | Cells | Trypanosoma brucei | 85 | 0.75 nM | Binding Buffer (20 mM NaxHyPO4 (pH 7.4),2 mM MgCl2,130 mM NaCl,5 mM KCl, 20 mM glucose,0.2 mM ß-mercaptoethanol) | 63.53% | 5' GGGAGACGAUAUUCGUCCAUUCGGGUGGCCCGUGUCUGAGCGGGGACGGCCACUUGAGCGCCGCUGUCCGACUGAAUUCUCGACC 3' | Homann and Goringer. "Combinatorial selection of high affinity RNA ligands to live African trypanosome." Nucleic Acid Research, 27(1999): 2006-2014. |
395 | ADP (ID# 262) | Small Organic | ADP | 85 | 1-5 µM | Selection Buffer ((50 mM HEPES [pH 7.4], 25 mM MgCl2, 150 mM NaCl) containing 10 g/ml tRNA) | 58.82% | 5' GGGCGACCCUGAUGAGCACACGAGGGGGAAACCCCGGACAAUCAGACACGGUGUUCGAAACGGUGAAAGCCGUAGGUUGCCCUUU 3' | Srinivasan et al. "ADP-Specific Sensors Enable Universal Assay of Protein Kinase Activity." Chemistry & Biology, 11(2004): 499-508. |
396 | Anti-NF-kB p65 (D1) (ID# 389) | Protein | Anti-NF-kB p65 | 85 | 149 nM | Selection buffer: 10mM HEPES at pH 7.5, 100mM NaCl, 1mM DTT | 49.41% | 5' GCAUGCAGUGUCUAUUCUCGAGUAGCGAUCGUUGAAGGGGUAUAAGGUUGGCAGAUCGCUAGCAUGCAACUGACUCGGAUAAGCA 3' | Wurster, S. E, and Maher, L. J. "Selection and characterization of anti-NF-kB p65 aptamers." RNA, 14(2008): 1037-1047. |
397 | Methylenedianiline (M1) (ID# 81) | Small Organic | Methylenedianiline | 87 | 450 nM | Binding buffer: 250 mM NaCl, 50 mM TrisHCl, 5 mM MgCl2 | 57.47% | 5' GGGAGACAAGAAUAAACGCUCAACUGCGAUCAGGGGUAAAUUUCCGCGCAGGCUCCACGCCGCUUCGACAGGAGGCUCACAACAGGC 3' | Brockstedt, U., et al. "In vitro evolution of RNA aptamers recognizing carcinogenic aromatic amines." Biochemical and Biophysical Research Communications, 313 (2004): 1004-1008. |
398 | Flavin Mononucleotide (AR5) (ID# 204) | Small Organic | FMN | 88 | N/A nM | Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl2 | 52.27% | 5' GGAUAAUAGCCGUAGGUUGCGAAAGCGACCCUGAUGAGCCUUAGGAUAUGCAUGAUGCAGAAGGACGUCGAAACGGUAGCGAGAGCUC 3' | Seetharaman et al. "Immobilized RNA switches for the analysis of complex chemical and biological mixtures." Nature Biotechnology, 19(2001): 336-341. |
399 | Signal Recognition Particle 19 (SRP19) (ID# 452) | Protein | Signal Recognition Particle 19 (SRP19) | 88 | 0.6 nM | 2X Gel Loading Buffer (equal volume used to quench reaction): 87 mM Tris base, 89 mM boric acid, 20% sucrose, 0.05% bromophenol blue, 0.05% xylene cyanol, 0.1% sodium dodecyl sulfate (SDS), 7.3 M urea, 1 mM EDTA. Additional 40 mM EDTA added. | 60.23% | 5' GGGAGACAAGAAUAAACGCUCAACACAGAACGCGGUCCCCACACAGGACAGGAGCCAGCCCCGGUUCGACAGGAGGCUCACAACAGGC 3' | Haraguchi, Y. Et al. Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA. Nucleic Acids Symposium Series, 2009, 53, 265-266. |
400 | Staphylococcus Aureus RNA Aptamer (ID# 593) | Cells | Staphylococcus Aureus | 88 | Tris-HCl, pH 7.5, 150 mM NaCl, 1.5 mM MgCl2, 2 mM DTT, 1 % BSA | 53.41% | Seung Ryul Han & Seong-Wook Lee, (2014). In vitro selection of RNA aptamer specific to Staphylococcus aureus. Ann Microbiol 64:883-885 DOI 10.1007/s13213-013-0720-z |