# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
31 Atrazine R12.23 Small Organic Atrazine 79 0.62 nM  500 µL of buffer consisting of 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride 50.63% 5’-TGTACCGTCTGAGCGATTCGTACGAACGGCTTTGTACTGTTTGCACTGGCGGATTTAGCCAGTCAGTGTTAAGGAGTGC-3’ Williams, R.; Crihfield, C.; Gattu, S.; Holland, L.; Sooter, L. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences, 2014, 15, 14332-14347. ISSN 1422-0067 doi: 10.3990/ijms150814332
32 Atrazine R12.28 Small Organic Atrazine 79 5.1 nM 500 µL of buffer consisting of 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride 50.63% 5’-TGTACCGTCTGAGCGATTCGTACCATTAGTGGGTGCTCCTTACCTGATGGTCATCTAGCCAGTCAGTGTTAAGGAGTGC-3’ Williams, R.; Crihfield, C.; Gattu, S.; Holland, L.; Sooter, L. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences, 2014, 15, 14332-14347. ISSN 1422-0067 doi: 10.3990/ijms150814332
33 HIV-1 Reverse Transcriptase (4.3) Protein HIV-1 RT 80 190 pM Binding buffer: 10 mM tris, 50 mM NaCl, and 1 mM EDTA (pH 7.5) 51.25% 5'-AGCAGCACAGAGGTCAGATGGCAGGTTTCGACGTACAATGCTATGGAGGCTTTATGATCGCCTATGCGTGCTACCGTGAA-3' Mosing et al. "Capillary Electrophoresis-SELEX Selection of Aptamers with Affinity for HIV-1 Reverse Transcriptase." Analytical Chemistry, 77 (2005): 6107-6112.
34 PCB72 (Aptamer 9.3) Small Organic Polychlorinated biphenyl 72 (PCB) 80 66 nM Binding Buffer (100 mM NaCl, 20 mM Tris–HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6) 57.50% 5'-AGCAGCACAGAGGTCAGATGCACTCGGACCCCATTCTCCTTCCATCCCTCATCCGTCCACCCTATGCGTGCTACCGTGAA-3' Mehta et al. "Selection and characterization of PCB-binding DNA aptamers." Analytical Chemistry 84(2012): 1669-1676
35 PCB106 (Aptamer 9.3) Small Organic Polychlorinated biphenyl 106 (PCB) 80 99 nM Binding Buffer (100 mM NaCl, 20 mM Tris–HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6) 57.50% 5'-AGCAGCACAGAGGTCAGATGCACTCGGACCCCATTCTCCTTCCATCCCTCATCCGTCCACCCTATGCGTGCTACCGTGAA-3’ Mehta et al. "Selection and characterization of PCB-binding DNA aptamers." Analytical Chemistry 84(2012): 1669-1676
36 Chloramphenicol-binding aptamer(c7) Small Organic chloramphenicol (cam) 80 0.766 µM 100mM NaCl, 20 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02 Tween 20, pH 7.6, r.t. 57.50% 5'-AGCAGCACAGAGGTCAGATGACTTCAGTGAGTTGTCCCACGGTCGGCGAGTCGGTGGTAGCCTATGCGTGCTACCGTGAA-3' J Mehta et al. In vitro selection and characterization of DNA aptamers recognizing chloramphenicol. J. Biotechnol. 155(2011): 361-369.
37 Neuropeptide Y (Clone 4.31) Peptide Neuropeptide Y (NPY) 80 300 nM Capillary Electrophoresis Separation Buffer: 20 mM Hepes (pH 7.3) with 10 mM NaCl 51.25% 5'-AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA-3’ S.D. Mendonsa and M.T. Bowser. In vitro selection of aptamers with affinity for neuropeptide Y using capillary electrophoresis. J. Am. Chem. Soc. 127(2005): 9382-9383.
38 Lysozyme (Apt 80)  Protein Hen egg white lysozyme and recombinant human lysozyme 80 2.8 nM Binding Buffer: 25 mM trizma base, 192 mM glycine, and 5 mM K2HPO4, pH 8.3. 57.50% 5'-AGCAGCACAGAGGTCAGATGGCAGGTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGCCCTATGCGTGCTACCGTGAA-3’ Zou M et al. (2012) The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer. Biosens. Bioelectron. 32: 148-154.
39 Lysozyme (Clone 1) Protein Hen egg white lysozyme 80 29-31 nM Tris Buffer: 20 mM Tris pH 7.6, 100 mM NaCl, 5 mM MgCl2 45.00% 5'-GGGAATGGATCCACATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGTTCACTGCAGACTTGACGAAGCTT-3’ Kirby R, et al. (2004) Aptamer-based sensor arrays for the detection and quantitation of proteins. Anal. Chem. 76: 4066 - 4075.
40 Lysergamine (M3.2) Protein Lysergamine, metergoline, and other small ergot alkaloids 80 44 nM Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 5 mM KCl, 2 mM MgCl2 ·6 H2O, 1 mM CaCl2·2 H2O. 62.50% 5'-AGCAGCACAGAGGTCAGATGGCGTCAGCCCCGATCGCCATCCAGGGACTCCCCCCTACTGCCTATGCGTGCTACCGTGAA-3’ ER Martin et al. (2012) Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids. Int. J. Mol. Sci. 13: 17138-17159.