# Aptamer Name Aptamer Target (General) Aptamer Target (Specific) Length Affinity Buffer GC Content Sequence Reference
31 Atrazine R12.23 Small Organic Atrazine 79 0.62 nM� 500 �L of buffer consisting of 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride 50.63% 5�-TGTACCGTCTGAGCGATTCGTACGAACGGCTTTGTACTGTTTGCACTGGCGGATTTAGCCAGTCAGTGTTAAGGAGTGC-3� Williams, R.; Crihfield, C.; Gattu, S.; Holland, L.; Sooter, L. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences, 2014, 15, 14332-14347. ISSN 1422-0067 doi: 10.3990/ijms150814332
32 Atrazine R12.28 Small Organic Atrazine 79 5.1 nM 500 �L of buffer consisting of 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride 50.63% 5�-TGTACCGTCTGAGCGATTCGTACCATTAGTGGGTGCTCCTTACCTGATGGTCATCTAGCCAGTCAGTGTTAAGGAGTGC-3� Williams, R.; Crihfield, C.; Gattu, S.; Holland, L.; Sooter, L. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences, 2014, 15, 14332-14347. ISSN 1422-0067 doi: 10.3990/ijms150814332
33 HIV-1 Reverse Transcriptase (4.3) Protein HIV-1 RT 80 190 pM Binding buffer: 10 mM tris, 50 mM NaCl, and 1 mM EDTA (pH 7.5) 51.25% 5'-AGCAGCACAGAGGTCAGATGGCAGGTTTCGACGTACAATGCTATGGAGGCTTTATGATCGCCTATGCGTGCTACCGTGAA-3' Mosing et al. "Capillary Electrophoresis-SELEX Selection of Aptamers with Affinity for HIV-1 Reverse Transcriptase." Analytical Chemistry, 77 (2005): 6107-6112.
34 PCB72 (Aptamer 9.3) Small Organic Polychlorinated biphenyl 72 (PCB) 80 66 nM Binding Buffer (100 mM NaCl, 20 mM Tris�HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6) 57.50% 5'-AGCAGCACAGAGGTCAGATGCACTCGGACCCCATTCTCCTTCCATCCCTCATCCGTCCACCCTATGCGTGCTACCGTGAA-3' Mehta et al. "Selection and characterization of PCB-binding DNA aptamers." Analytical Chemistry 84(2012): 1669-1676
35 PCB106 (Aptamer 9.3) Small Organic Polychlorinated biphenyl 106 (PCB) 80 99 nM Binding Buffer (100 mM NaCl, 20 mM Tris�HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6) 57.50% 5'-AGCAGCACAGAGGTCAGATGCACTCGGACCCCATTCTCCTTCCATCCCTCATCCGTCCACCCTATGCGTGCTACCGTGAA-3� Mehta et al. "Selection and characterization of PCB-binding DNA aptamers." Analytical Chemistry 84(2012): 1669-1676
36 Chloramphenicol-binding aptamer(c7) Small Organic chloramphenicol (cam) 80 0.766 �M 100mM NaCl, 20 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02 Tween 20, pH 7.6, r.t. 57.50% 5'-AGCAGCACAGAGGTCAGATGACTTCAGTGAGTTGTCCCACGGTCGGCGAGTCGGTGGTAGCCTATGCGTGCTACCGTGAA-3' J Mehta et al. In vitro selection and characterization of DNA aptamers recognizing chloramphenicol. J. Biotechnol. 155(2011): 361-369.
37 Neuropeptide Y (Clone 4.31) Peptide Neuropeptide Y (NPY) 80 300 nM Capillary Electrophoresis Separation Buffer: 20 mM Hepes (pH 7.3) with 10 mM NaCl 51.25% 5'-AGCAGCACAGAGGTCAGATGCAAACCACAGCCTGAGTGGTTAGCGTATGTCATTTACGGACCTATGCGTGCTACCGTGAA-3� S.D. Mendonsa and M.T. Bowser. In vitro selection of aptamers with affinity for neuropeptide Y using capillary electrophoresis. J. Am. Chem. Soc. 127(2005): 9382-9383.
38 Lysozyme (Apt 80)� Protein Hen egg white lysozyme and recombinant human lysozyme 80 2.8 nM Binding Buffer: 25 mM trizma base, 192 mM glycine, and 5 mM K2HPO4, pH 8.3. 57.50% 5'-AGCAGCACAGAGGTCAGATGGCAGGTAAGCAGGCGGCTCACAAAACCATTCGCATGCGGCCCTATGCGTGCTACCGTGAA-3� Zou M et al. (2012) The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer. Biosens. Bioelectron. 32: 148-154.
39 Lysozyme (Clone 1) Protein Hen egg white lysozyme 80 29-31 nM Tris Buffer: 20 mM Tris pH 7.6, 100 mM NaCl, 5 mM MgCl2 45.00% 5'-GGGAATGGATCCACATCTACGAATTCATCAGGGCTAAAGAGTGCAGAGTTACTTAGTTCACTGCAGACTTGACGAAGCTT-3� Kirby R, et al. (2004) Aptamer-based sensor arrays for the detection and quantitation of proteins. Anal. Chem. 76: 4066 - 4075.
40 Lysergamine (M3.2) Protein Lysergamine, metergoline, and other small ergot alkaloids 80 44 nM Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 5 mM KCl, 2 mM MgCl2��6 H2O, 1 mM CaCl2�2 H2O. 62.50% 5'-AGCAGCACAGAGGTCAGATGGCGTCAGCCCCGATCGCCATCCAGGGACTCCCCCCTACTGCCTATGCGTGCTACCGTGAA-3� ER Martin et al. (2012) Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids. Int. J. Mol. Sci. 13: 17138-17159.